The bioactive compounds of barberry such as water-soluble vitamins are used in medical and food industries. In this study, the effect of different extraction conditions and various process conditions were studied on water-soluble vitamins profile. The extraction conditions included varieties (species) are of fruits (B. integerrima: A, B. vulgaris: P), solvents (Water: W, Ethanol: E), light (Presence: L, Absence: T), pH (3 and 1. 5), and temperature (25 and 50º C). The process conditions included heating (95 and 80° C), chilling (ref 1, 2 months), freezing (con), microwave (mic) and gamma irradiation (γ at doses of 0. 5, 1, 2. 5, 5, 7. 5, 10 kGy). The highest and lowest amounts of water-soluble vitamins in various extracts of different extraction conditions were respectively as follows: vitamin C in AWL1. 550 (highest) and PEL350 (lowest); B5 in PWL1. 550 and AEL1. 525, AWL1. 550; B6 in AWT350, and AEL1. 550; B1 in PET350 and PET1. 550; folic acid in AET350 and PWT350; biotin in AEL350 and AEL1. 550; B2 in AET350 and PWL350 extracts. The highest and lowest amounts of water-soluble vitamins in various process conditions were respectively as follows: vitamin C (Acon 460 (highest), Aref1 146. 87 (lowest), Pcon 242. 96 (highest), Pmic 21. 52 (lowest)), B5 (Aγ 0. 5 2919. 18, A95 1312. 42, Pγ 10 3110. 88, Pref2 1051. 52), B6 (Acon 36. 30, Aγ 7. 5 21. 04, Pγ 10 12. 70, P95 8. 73), B1 (Acon 2113. 00, Aref2 965. 09, Pcon 2298. 15, Pγ 10 217. 76), folic acid (Aγ 0. 5 1700. 38, Aγ 10 947. 11, Pcon 104. 78, Pγ 10 in B. vulgaris after processing was not found), biotin (Amic2267. 58, Aγ 101404. 63, Pcon324. 72, Pγ 2. 5118. 33), and B2 (Aγ 0. 5586. 43, Aγ 10274. 34, Pcon297. 19, Pγ 106. 79).