Introduction: Cervical cancer is one of the important health problems and one of the common gynecologic disease with high mortality rate. This study was performed with the aim to compare the Cervix-Brush-Combi with Cervix- Brush for collection of cervical cells in Pap smear.Methods: In this quasi experimental study, 100 women referred to health care center No. 2 in east of Ahvaz were randomly divided into two group of code 1 (firstly, Cervix-Brush-Combi and then Cervix- Brush) and code 2 (firstly, Cervix- Brush and then Cervix-Brush-Combi). 200 samples were prepared from Endocervix and exocervix, and then, Brush''s tip was removed from the trunk and was thrown into the vial with an alcohol base. After the closure of the vial, 20-15 times was shaken and sent to the laboratory. Data was analyzed by SPSS (version 16), and independent t-test and chi-square test. PResults: Mean number of exocervical and endocervical cells collected by Cervix-Brush-Combi were 63.3±48.0 and 636.3±545.5, respectively and collected by Cervix- Brush were 70.0±62.1 and 607.2±378.8, respectively. Independent t–test and chi-square test were not found significant difference between two tools.Conclusion: Cervix-brush combi tool has the same efficiency and effectiveness in terms of collection of cervical cells compared to the cervix brush tool.