Introduction: Elastomeric chains are used for space closure in orthodontics. In this study the effect of sodium fluoride (NaF) mouth rinse on the elastic properties of chains was evaluated.Materials & Methods: In this in vitro study, 6-linked sections of transparent open chains of American Orthodontics and Dentaurum companies were tested in two groups. In the first group, chains were only stored in artificial saliva. Chains of the second group were daily immersed in 0.05% NaF rinse for 1 minute, then in a mixture solution of saliva and NaF for 30 minutes, and then were stored in saliva. At time intervals of initial, 1,24 hours, 1,2,3 weeks, displacement of each specimen to obtain forces of 150g and 300g was determined with Zwick machine in the tensile test. Also at the same intervals, after the specimens were stretched to 100% of their original length in the degradation test, the applied force was measured. Results: Specimens required more displacement to achieve forces of 150g and 300g in the mixture solution than saliva, but the difference was only significant for the 300g force (P=0.020). Force degradation of chains was not statistically different between the two solutions. American Orthodontics chains showed more force degradation than Dentaurum (P<0.001).Conclusion: Daily use of NaF mouth rinse did not cause considerable increase in the displacement of chains to achieve conventional orthodontic forces (150g) and did not affect the force degradation of selected chains. Because of their higher force degradation, American Orthodontics chains should be changed at shorter intervals than Dentaurum ones.