In order to estimate the biomass of demersal resources in Khouzestan and Boushehr waters (North-West Persian Gulf), a research survey was carried out using R/V Ferdows-1 covering the depths 10-20, 20-30 and>30m. Study area was stratified into 2 strata (A, B) and 32 stations were selected randomly and sampled by Swept Area method. In substrata A1, B1, B2 and B3, 44, 43, 54 and 36 species were identified, respectively. Catch Per Unit Area and biomass in substrata A1, B1, B2 and B3 were 2396, 3102, 4942, 7904 kg/nm2 and 1198, 1292, 2471, 3952 kg, respectively. The maximum biomass was recorded for Upeneus sulphureus (1269kg) and Nemipterus Japonicus (857kg) and the minimum for Apogon sp. (2.23kg) and Scorpion sp. (4.18kg). Total biomass in the area was estimated at 9015 kg of which 6491 kg was non-commercial. A decrease of fishing effort should seriously be taken into consideration.