Among the accidents which endager human health, burns are considered as the most severe ones. When skin is damaged, pathogenic agents attack the body and after a short time burn wounds are infected by resistant microorganisms, that is the most important cause of the mortality in burned patients. The present study was carried out to determine the outbreak rate of infection in burn wounds of the hospitalized patients, as well as, to access a proper treatment procedure based on pathogenic factors which cause infection.This descriptive study was done within six months on all the hospitalized patients (82) in the burn ward of Shohadaye Ashayer Hospital in Khorramabad (Iran).The instrument for collecting data was a questionnaire and the results of the tissue cultures which were taken from the wounds in two phases in the sterile condition, the first time one week and the second time two weeks after burning. The size of samples was one centimeter .The tissue cultures were processed in the mere unaerobic and aerobic in the temperature of 37°C. Samples with 105 colony per gr tissue were considered as infected samples, in addition the type of microoganisms developed in the samples were indentified . Also items such as: age, gender, type of bum, and the relation of some of them with the infection were identified. Findings showed that 33 patients had 30-40% of burned surfaces: The tissue culture was positive. Among these, 72% had infected with pseudomonas aeroginosa and the rest had infected with E.Coli, staphylococcus, candidacies, and proteous .Among all the patients, 5 persons died. These patients had more than 35% burned surface and their wounds were infected. The average burned surface of the infected patients was 43.6% and the average burned surface among patients without infection was 25%. The statistical operation (pV=O) showed that there is a significant difference between the percentage of burned surface and the occurrence of infection.The most common factor coursing infection in the burn wounds was pseudomonad that matches with the results of the other studies. There was also a significant relationship between the percentage of burned surface and-the occurrence of infection Regarding the much attention and notice to the identification of infection using tissue culture, we suggest that the treatment of infection in burned patients should be done based on this test and the type of microorganisms cousing infection.