The effects of N supply, growing medium and transplanting time on the photosynthesis, growth and macronutrients level of two olive (Olea europaea L.) cultivars ('Arbiquina' and 'Roughani') Were studied. In two propagation times (spring and autumn), the rooted plants were grown in plastic pots containing perlite: sand (1: 1) or soil: sand: organic matter (1: 1: 1) mixtures and were irrigated with different amounts and forms of nitrogen for 9 months. The result of this study indicated that olive can use both the nitrate and ammonium forms of N. However, high level of nitrogen, especially ammonium form, is deleterious to the photosynthesis and growth of olive nursery plants, therefore, depended on olive cultivars, addition of small amounts of NH4 to N03 solution (12 meq lit-1), increases of the photosynthesis and growth (4, 6 meq lit-1 NH4). for 'Arbiquina' and 'Roughani', respectively. Addition of NH4 to nutrient solutiond influences not only the rate of growth but also the absorption of other ions. Absorption of NH4 by plants, as compared to N03, decreases K and increases N and P uptake. The mean effect of the cultivar on the organs concentration of nutrients was significant and high N and P and high K use efficiency was seeing in 'Arbiquina' and 'Roughani' cultivars, respectively. On the other hand, olive growth and photosynthesis affected by growing media and transplanting time, so that, the soil media and spring time were the best for these purposes.