COMPARISON OF DIFFERENT CLASSICAL AND MOLECULAR METHODS FOR IDENTIFICATION OF SELF- COMPATIBLE AND SELF- INCOMPATIBLE ALMOND PROGENIES

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KAMALI K.; EBADI A.; FATTAHI MOGHADAM M.R.; NAGHAVI M.R.; IMANI A.

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Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2009 | Volume:10 | Issue:1 Page(s): 53-70

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Title

COMPARISON OF DIFFERENT CLASSICAL AND MOLECULAR METHODS FOR IDENTIFICATION OF SELF- COMPATIBLE AND SELF- INCOMPATIBLE ALMOND PROGENIES

Author(s)

Keywords

ALMONDQ2

FLUORESCENT MICROSCOPYQ3

NEPHGEQ3

SELF- COMPATIBILITYQ2

SELF- INCOMPATIBILITYQ3

PCRQ3

STYLAR RIBONUCLEASES ANALYSISQ3

Abstract

Self -incompatibility is one of the major problems in ALMOND commercial cultivars. To solve that, many breeding programs have been conducted. In this research, late blooming, self- compatible cultivar of 'G-2-25' (S11Sf) as a female parent was crossed with self- incompatible cultivars of 'Desmayo Largueta' (S1S25) and 'Marcona' (S11S12) both as male parents. Progenies were evaluated for their S- allele genotype by FLUORESCENT MICROSCOPY, analyzing of stylar ribonucleases by NEPHGE and PCR methods. The results showed that all different methods including FLUORESCENT MICROSCOPY, NEPHGE and PCR, could differentiate self- compatible from self- incompatible genotypes and their results confirmed each other. The ratio of S- allele genotypes in population obtained from crossing of 'Marcona' and 'G-2-25' was according to expected mendelian ratio. However, in crossing between 'Desmayo Largueta' and 'G-2-25', only genotypes of S1S11 (21%), S1Sf (53%) and S25Sf (26%) were obtained and there was no S11S25 genotype among progenies. This situation may be related to lethal gene effects, or certain reciprocal effect among S- alleles.

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APA: Copy

KAMALI, K., EBADI, A., FATTAHI MOGHADAM, M.R., NAGHAVI, M.R., & IMANI, A.. (2009). COMPARISON OF DIFFERENT CLASSICAL AND MOLECULAR METHODS FOR IDENTIFICATION OF SELF- COMPATIBLE AND SELF- INCOMPATIBLE ALMOND PROGENIES. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 10(1), 53-70. SID. https://sid.ir/paper/80711/en

Vancouver: Copy

KAMALI K., EBADI A., FATTAHI MOGHADAM M.R., NAGHAVI M.R., IMANI A.. COMPARISON OF DIFFERENT CLASSICAL AND MOLECULAR METHODS FOR IDENTIFICATION OF SELF- COMPATIBLE AND SELF- INCOMPATIBLE ALMOND PROGENIES. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2009;10(1):53-70. Available from: https://sid.ir/paper/80711/en

IEEE: Copy

K. KAMALI, A. EBADI, M.R. FATTAHI MOGHADAM, M.R. NAGHAVI, and A. IMANI, “COMPARISON OF DIFFERENT CLASSICAL AND MOLECULAR METHODS FOR IDENTIFICATION OF SELF- COMPATIBLE AND SELF- INCOMPATIBLE ALMOND PROGENIES,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 10, no. 1, pp. 53–70, 2009, [Online]. Available: https://sid.ir/paper/80711/en

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