This experiment was conducted to study the effect of south-west climatic variation on morphological characteristics, vitamin C, and fatty acids of dog rose (Rosa canina L.) fruits. The fruits were collected from five different regions in south-west of Iran. Results showed that length, thickness, flesh percentage, weight, and flesh to seed ratio of fruit were significantly different at 5% level but width and length to width of fruit were not significantly different. Vitamin C contents of fruits were determined by HPLC. Differences among studied regions regarding the vitamin C were significant. The largest and lowest of vitamin C content was obtained from Gerd Bishe (1384.4 mg 100-1 g FW) and Kiar (721.64 mg 100-1 g FW) respectively. Oil was extracted using Soxhlet apparatus and analyses of fruit fatty acids were carried out by gas chromatography (GC). The results confirmed the presence of five dominant fatty acids in fruits of dog rose, including: linoleic acid, oleic acid, linolenic acid, palmitic acid, and stearic acid, respectively. Differences among oleic acid, palmitic acid, and stearic acid quantity in the studied regions were significant, whereas differences among linoleic and linolenic acid quantity was not significant. The results of the present study showed that climatic factors affect quantity of vitamin C, fatty acid, and morphological characteristics of dog rose.