Anatomical Sciences Journal
Year:2003 | Volume:1 | Issue:4|Papers Count:9

Purpose: The aim of this study was to evaluate the morphological changes of vaginal epithelium after hyperstimulation and daily injection of progesterone followed ovarian hyperstimulation.Materials and Methods: Forty 8-10 week old virgin female mice were selected and divided in four groups. Two experimental groups were hyperstimulated using ip injection of 10 Iu of human menopasula gonadotropin hormone (hMG) followed with another injection of 10 Iu human chorionic gonadotropic hormones (hCG) 48 hrs later. After superovulation of mice, progesterone injections were performed daily subcutaneously (1 mg/mouse) in another experimental and or hyperstimulated groups. On the days three and four after hCG injection, the samples were aparted from the vaginal septum and processed for morpgological studies. All experimental and control groups were rendered pseudopregnant artificially.Results: Our results showed that the height of epithelium was increased on the fourth day of hCG injection in hyperstimulated groups in comparison with control group. Superficial layer had a lot of cornified cells. The height of vaginal epithelium in the progesterone injected and hyperstimulated-progesterone injected groups in comparison with the hyperstimulated was decreased.Conclusion: Thus, the progesterone injection after hyperstimulation can cause changes in morphology of mouse vaginal epithelium which can influence the function and secretion of the epithelium.

Purpose: The reaction of compact bone of diaphyse of tibia and it's bone marrow to low power laser radiation was investigated.Materials and Methods: Ten adult male rabbits were divided randomly into experimental and control groups. Low-power He-Ne laser (36 J/cm2) were radiated into a fixed point of tibia daily for 14 consequative days. Rabbits of control group did not receive laser. At the end of the period rabbits were killed by chloroform and a sample from each rabbit was extracted. Samples were fixed in formalin saline and were decalcified by EDTA, and prepared for light microscopic study. Sections were stained by Hematoxyline and Eosine method and examined descriptively and quantitatively.Results: In control group, compact bone is composed of lamellar bone which includes many regular haversian systems. In experimental group lamellae of haversian systems were irregular and in some of haversian chanals, hemopoitic activities were observed. Periosteum of experimental group was thicker than control group. There were more blood cells in the central medulary chanal of experimental group than control group.Conclusion: He-Ne low-power laser radiation increased periosteal thickness and hemopoitic activity of rabbit's Tibia.

Purpose: To evaluate the effect of compund of olea europea L. and myrtus communis L. on burn wound (type 2) healing.Materials and Methods: 48 female rats were studied through dividing them to 4 groups of 12 rats. First group were choosen for a period of 7 days of treatment, second group for the period of 14 days treatment, the third group for the period of 21 days, and the fourth group for period of 28 days of treatment.Each group were divided in to 3 groups 1- control group, 2- Silver sulphadiazin, 3- myrtus oil group After general anesthezia with ketamin and xylazin (i.p) an area of the back of rats was burned with red-hot 50 Rials coin and similar grade II burns were created. Control grouop received no medication. In the second and third groups, the surface of the wound were covered daily with sulphadiazine and myrtus and respectively.At the end of each period rats were killed by Ether were samples were taken from the wound region, and were histologically assessed. Then, the samples were coloured studied with hematoxillin and Eosin and studied quantitatively.Results: Results showed that after 4 weeks the healing of burn wounds in the group receiving myrtus communis L. and olea europeal L. oil and silver sulphadiazin was faster, better than the control group. There were no significant difference between the group receiving myrtus oil and silver sulphadiazin, but reduction in scar tissue after healing in the group treated by myrtus oil comparing with other groups.Conclusion: Administration of topycal myrtus oil once a day in second degree burn wounds enhances the healing comparing to control group. Nearly similar results were observed in the silver sulphadiazin group comparing with myrtus oil group.

Purpose: This study is designed to determine the sex difference in volume of precentral cortex of left hemisphere in right-handed normal subjects and the right-handed subjects which are suffering from Alzheimer and Parkinson diseases.Materials and Methods: This study was performed on 72 normal human brains (38 males, 34 females), 11 human brains suffering from Alzheimer (4 males, 7 females), and 13 brains suffering from Parkinson (9males, 4 females). The age range between males and females in each group was similar. The brains were fixed in 4% formalin.The brainstem was removed and two hemispheres were separated by a midsagittal section. The right hemisphere was used for neuropathological diagnosis and the left one was used for morphometrical analyses. Volume esimation of the precentral cortex of left hemisphere is performed on photographs of 5mm serial coronal sections of this hemisphere using the Cavalieri's principle and point counting. The results were analysed by t-student and non-parametical Mann-Whitney Statistical tests.Results: The volume of left precentral cortex had no significant sexual difference in any of three groups (normal, Alzheimer, and Parkinson groups). There was only a tendency for this parameter to be larger in males than females in normal and Parkinson groups.Conclusion: Although the volumes of left hemisphere and its frontal cortex are larger in normal men, there is no significant difference between two sexes in volume of precentral cortex. Therefore, the sexual dimorphism in volume of frontal cortex in normal group may be due to other subdivisions of this lobe, except than precentral gyrus.

Purpose: The study of effects of mercuric chloride on cells arrangment of diencephalon region (thalamus and hypothalamus) of Rat embryos.Materials and Methods: 36 Rat embryos from Sprague Dawley strain were selected and devided in to six groups. In three groups (control groups) normal saline solution and in three groups (experimental groups) 2mg/kg mercuric chloride were injected intraperitonealy in eighth, ninth, tenth days of gestation seperately. Then, on the day 15 of pregnancy, embroyos were removed. After fixation and tissue passage procedures, some slides of embroys were prepared with 5 microns thickness, staind by H&E method, and then studied by light microscope. Cell count has done by eye-piece and SPSS method, LSD, Duncan tests were used.Results: In neuroblasts of thalamus and hypothalamus region in experimental groups cellular disarrangement observed and nuclei were laid on different orientation against nural tube lumen, cell concentration decreased and extracellular space was increased, cell death and degeneration was seen.Conclusion: Mercuric chloride affected on neuroblasts of thalamus and hypothalamus region and caused loss of neuroblasts. This could disorder normal development of neural tube and act as a theratogen ingestation period.

Purpose: This study was done for determining of head dimensions and head shapes. The purpose of the present study was to determine head dimensions and shapes.Materials and Methods: This discriptive and cross sectional study was conducted on normal 17-20 year-old native Fars and Turkman ethnic groups in Gorgan. Martin saler scalope was used to measure cephalic length and width, then cephalic index was conducted. Based on cephalic index, the cephalic form was classified as: Brachycephaly, Hyperbrachycephaly, Dolicocephaly and Mesocephaly.Results: the mean (ISD) of cephalic index in Turkman group and native Fars were 80.4±4 and 84.8 ± 6.9 respectivly. Hyperbrachycephaly was dominant (52%) in native Fars whereas the brachycephaly was dominant (42.14%) in Turkman. Dolicocephaly was the least prevalen type in both groups.Conclusion: This research indicated that there was a significant difference in head shapes between two groups. This may be due to possible effect of ethnis factor on the diversity of head shapes.

Purpose: Manganese, are of the elements plenty & which is found in nature, is widely used in Agricalture and in dustry.For years, toxicity of magnasium and its derivatives has been proven yet the findings are not valid enough to overgeneralize the result to human.In the present study the effect of neurotoxicity of manganese chloride on the mouse cortex was investigated.Materials and Methods: Pregnant mice were devided into too groups: one control, and the other experimental. In the experimental group 45 mg/kg MnCl2 were injected intrapertioneally on the seventh, eigth and ninght of gestation period and the distilled water was injected into control group. Embryos were then removed on the day 15. Embryos were weighted and fixed in Buin solution. After tissue passage procedures, samples of 5 µ were prepared sections and stained with Hematoxiline, eosine. Then the samples were evaluated.Results: Macroscopic measurements showed that embryos weights on the days 7, 8 and 9 significantly decreased in experimental group comparing with controls. Thickness of corpus striatum showed significant reduction on the day 7 & 8 in experimental group comparing with control. In experimental group gliosis were also observed.Conclusion: The results indicated that manganese chloide had toxic effects. Therefore, pregnants should not be exposed to high levels MnCl2.

Purpose: The aim of this study was to evaluate the effect of age on the capacity of liver and epiderm of adult and weanging rats in transformation of Benzo (α) Pyrene.Materials and Methods: In a metabolic activiation assay system, cytochorome P-50 (from microsomal fraction) catalyses the formation of reactive epoxide of BaP which can then interact with exogenous DNA The capacity of cytochrome P-450 for BαP-DNA adducts formation from different organs are adult rat live>>> adult rat skin>> weanging rat liver> weanling rat skin. With the addition of cytosol (the source of glutathione S transferase (GST) to the microsome-mediated system an In vitro detoxification model has been established.Results: The results obtained by the addition of different cytosolic samples are shown in several tables in the text. These data show that isolation of subcellular fractions from young rats are less efficient in the biotransformation of BαP and detoxification by GSH is a passive process that depends on activation process. Also metabolic activation of BaP with cytochorome P-450 is not the first degree with glutathione detoxification. However, the results obtined In vitro do not reflect the exact change In vivo.