Phytoseiid mites are the most important and studied natural enemies of pest mites. Phytoseiulus plumifer (Acari:Phytoseiidae) is one of the most important natural enemies of spider mites in Iran. This investigation was carried out to examine the effects of the prey species and their different densities on functional response of P. plumifer. Adults of the predatory mite after collection from botanic garden (Karaj) reared on Ficus leaves. The 24-h leaf disc experiments conducted at laboratory condition (27±1oC, 50±10% RH, 16:8 L:D photoperiod) on apple leaves as host plant. The larvae of Tetranychus urticae and Amphitetranychus viennensis (Acari: Tetranychidae) were offered as prey at the following densities 2, 4, 6, 8, 16, 25 , 35 and 2, 4, 6, 8, 16, 25 , 35, 45 and 50 for T. urticae and A. viennensis respectively. The results indicated that the predatory mite exhibited type III and II functional response against the T. urticae and A. viennensis (Acari: Tetranychidae) respectively. After determining the type of functional response parameters must be estimated. The attack coefficient (a) and the handling time (Th) for A. viennensis were 0.0638 ± 0.0164 and 0.9841±0.1494 h, respectively. For T. urticae, b was 0.0021±0.0003 and 0.9696±0.1185h, respectively. Maximum predation on T. urticae and A. viennensis were estimated 24.75 and 24.39 larvae per day.