EFFECTS OF GROWTH REGULATORS ON IN VITRO CALLOGENESIS AND REGENERATION OF BLACK CUMIN (NIGELLA SATIVA)

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HOSEINPANAHI S.; MAJDI M.R.; MIRZAGHADERI GH.

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ISSN: 2588-4824

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Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2016 | Volume:24 | Issue:2 Page(s): 232-242

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Title

EFFECTS OF GROWTH REGULATORS ON IN VITRO CALLOGENESIS AND REGENERATION OF BLACK CUMIN (NIGELLA SATIVA)

Author(s)

HOSEINPANAHI S. | MAJDI M.R. | MIRZAGHADERI GH. | Issue Writer Certificate

Keywords

AUXINQ2

BLACK CUMINQ2

CALLOGENESISQ2

CYTOKININQ2

REGENERATIONQ1

TISSUE CULTUREQ2

Abstract

Black cumin (Nigella sativa) is one of the most important medicinal plants belongs to Ranunculaceae. In spite of its significance in pharmaceutical industry, TISSUE CULTURE and micropropagation of the species is not well studied yet. Hence, based on the issue we aimed to study its CALLOGENESIS and REGENERATION on leave explants of the species. In vitro provided explants of leave were cultured on MS medium supplemented with NAA (0.5, 1, 1.5 mgL-1) and BAP (0.2, 0.5, 1 mgL-1). Half MS medium supplemented with 0.5, 1 and 1.5 mgL-1 NAA and IBA were used for rooting the regenerated shoots. Results showed that higher percentage of CALLOGENESIS was observed on MS medium supplemented with 0.5 mgL-1 NAA and 0.5 mgL-1 BAP. Shooting was observed on MS medium (1mgL-1 BAP) and rooting on half MS (1 mgL-1 NAA), respectively. Results indicated that CALLOGENESIS, shooting and rooting of BLACK CUMIN are highly affected by hormonal combination levels.

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APA: Copy

HOSEINPANAHI, S., MAJDI, M.R., & MIRZAGHADERI, GH.. (2016). EFFECTS OF GROWTH REGULATORS ON IN VITRO CALLOGENESIS AND REGENERATION OF BLACK CUMIN (NIGELLA SATIVA). IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 24(2), 232-242. SID. https://sid.ir/paper/104171/en

Vancouver: Copy

HOSEINPANAHI S., MAJDI M.R., MIRZAGHADERI GH.. EFFECTS OF GROWTH REGULATORS ON IN VITRO CALLOGENESIS AND REGENERATION OF BLACK CUMIN (NIGELLA SATIVA). IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2016;24(2):232-242. Available from: https://sid.ir/paper/104171/en

IEEE: Copy

S. HOSEINPANAHI, M.R. MAJDI, and GH. MIRZAGHADERI, “EFFECTS OF GROWTH REGULATORS ON IN VITRO CALLOGENESIS AND REGENERATION OF BLACK CUMIN (NIGELLA SATIVA),” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 24, no. 2, pp. 232–242, 2016, [Online]. Available: https://sid.ir/paper/104171/en

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