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Information Journal Paper

Title

EFFECT OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND REGENERATION OF SUGARCANE VARIETY CP73-21

Pages

  47-54

Abstract

 In this study, in order to disinfect INITIAL LEAVES of the sugarcane (SACCHARUM OFFICINARUM) variety CP73-21, five treatments were studied in a completely randomized design (CRD) with six replications.Sequential applications of 25% sodium hypochlorite for 10 minutes and 0.1% mercuric chloride for 30 seconds had the best results on disinfection of INITIAL LEAVES. Six callus induction treatments were evaluated through a factorial experiment based on CRD with four replications. Murashig and skoog (MS) based medium supplemented with 3 mgl-1 2,4-D along with 0.1 mgl-1 and 0.3 mgl-1 BAP respectively, had the best results for callus induction of INITIAL LEAVES. Regeneration of calli was studied using five treatments in a completely randomized design with four replications. Hormone- free modified MS (including casein hydrolyzate) and also modified MS with 1 mgl-1 BAP and 0.2 mgl-1 NAA were more efficient on regeneration. ROOT FORMATION was studied using five treatments in a completely randomized design with four replications. Half- strength MS medium supplemented with 0.01 mgl-1 IBA was found as the best for root induction treatment. The regenerants passed different stages of ACCLIMATIZATION successfully.

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    APA: Copy

    SADAT, SH., BIHAMTA, M.R., & EMAM, S.E.. (2008). EFFECT OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND REGENERATION OF SUGARCANE VARIETY CP73-21. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 15(5), 47-54. SID. https://sid.ir/paper/9612/en

    Vancouver: Copy

    SADAT SH., BIHAMTA M.R., EMAM S.E.. EFFECT OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND REGENERATION OF SUGARCANE VARIETY CP73-21. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2008;15(5):47-54. Available from: https://sid.ir/paper/9612/en

    IEEE: Copy

    SH. SADAT, M.R. BIHAMTA, and S.E. EMAM, “EFFECT OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND REGENERATION OF SUGARCANE VARIETY CP73-21,” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 15, no. 5, pp. 47–54, 2008, [Online]. Available: https://sid.ir/paper/9612/en

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