EFFECTS OF GENOTYPE AND GROWTH REGULATORS ON CALLUS INDUCTION IN ROSA DAMASCENA MILL

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TABAEI AGHDAEI S.R.; MIRJANI L.; EMAM M.; ASAREH M.H.; GHAMARI ZARE A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2007 | Volume:15 | Issue:3 (29) Page(s): 222-230

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Title

EFFECTS OF GENOTYPE AND GROWTH REGULATORS ON CALLUS INDUCTION IN ROSA DAMASCENA MILL

Author(s)

Keywords

CALLUS INDUCTIONQ3

EXPLANTQ3

PCAP (P-CHOLORO PHENOXY ACETIC ACID) AND ROSA DAMASCENA MILLQ3

Abstract

Rosa damascena Mill. as a most important aromatic plants is the best commercial species of the fragrant roses in Iran and many countries. To study CALLUS INDUCTION and proliferation in Rosa damascena Mill., axillary buds of five genotypes collected from different parts of Iran was used as EXPLANTs. These were cultured on MS medium supplemented with growth regulator(s) and solifiled with 0.7% (w/v) agar, at 25±1oC. Different concentrations of 2,4-D (2, 3 ,4 ,5 ,6 mg/l), IBA (0.1 mg/l), NAA (5 ,10 mg/l), PCPA (3, 5 ,7 ,9 ,10 mg/l) Kinetin (0.1, 1 mg/l) and BAP (1, 2mg/l) were used. Genotypes showed significant differences (P<0.01) for CALLUS INDUCTION, Also, highest CALLUS INDUCTION was observed in genotypes of Kashan cultured on MS cultured medium contained 1 mg/l Kinetin and 5 mg/l PCPA. The results emphasized on genotype, kind and concentration of growth regulators as key factors in callogenensis and proliferation of Rosa damascena.

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