Callus induction and regeneration of different accessions for genetic variation of Rosa damascene Mill.

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MIRJANI L.; EMAM M.; TABAEI AGHDAEI S.R.

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Journal: Journal of Molecular and Cellular Researches Year:2018 | Volume:31 | Issue:1 Page(s): 55-66

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Title

Callus induction and regeneration of different accessions for genetic variation of Rosa damascene Mill.

Author(s)

MIRJANI L. | EMAM M. | TABAEI AGHDAEI S.R. | Issue Writer Certificate

Keywords

Rosa damascenes MillQ1

OrganogenesisQ2

CallusQ2

accession and Growth regulatorsQ2

Abstract

Rosa damascenes Mill. is a most important aromatic plant that it has pharmacies properties'. In this research regeneration of 18 different accessions was studied for genetic diversity production. Callus induction was achieved on leaf, petiole, stipule and filament sections of these accessions. Culture of explants was done on semi solid MS medium supplemented with growth regulator(s) in different concentrations of 2, 4-D, IBA, NAA, PCPA, Kinetin and BAP. Total Callus induction about each accession was measured and these data were analyzed at randomized complete design by using of SPSS software (2006). Averages were compared by Duncan's multiple rang test. Genotypes showed significant differences (P<0. 01) for Callus induction. Also, lowest and highest Callus induction was observed repeatedly in genotypes of Azerbaijan ( western and eastern) and Isfahan 6 that last, cultured on MS medium contained 1 mg/l Kinetin and 5 mg/l PCPA. The media such as B5, DKW and MS with different ranges of growth regulators were used for regeneration. Within 18 accessions, regeneration of Callus was done at stem explants of Isfahan 4 accession in MS medium with 3mg/l BA. The results emphasized on genotype, kind of explants and concentration of growth regulators as key factors in regeneration of Rosa damascene and optimal application of these factors was necessary for soma clonal variation followed by Organogenesis and regeneration from Callus.

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APA: Copy

MIRJANI, L., EMAM, M., & TABAEI AGHDAEI, S.R.. (2018). Callus induction and regeneration of different accessions for genetic variation of Rosa damascene Mill.. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 31(1 ), 55-66. SID. https://sid.ir/paper/248419/en

Vancouver: Copy

MIRJANI L., EMAM M., TABAEI AGHDAEI S.R.. Callus induction and regeneration of different accessions for genetic variation of Rosa damascene Mill.. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2018;31(1 ):55-66. Available from: https://sid.ir/paper/248419/en

IEEE: Copy

L. MIRJANI, M. EMAM, and S.R. TABAEI AGHDAEI, “Callus induction and regeneration of different accessions for genetic variation of Rosa damascene Mill.,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 31, no. 1 , pp. 55–66, 2018, [Online]. Available: https://sid.ir/paper/248419/en

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