STUDY OF DROUGHT TOLERANCE IMPROVEMENT IN ALFALFA (MEDICAGO SATIVA L.) USING SOMACLONAL VARIATION

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ASKARI H.; SAFARNEZHAD ABAS; KAZEMI TABAR S.K.; HAMIDI H.

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Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2004 | Volume:12 | Issue:2 Page(s): 117-130

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Information Journal Paper

Title

STUDY OF DROUGHT TOLERANCE IMPROVEMENT IN ALFALFA (MEDICAGO SATIVA L.) USING SOMACLONAL VARIATION

Author(s)

ASKARI H. | SAFARNEZHAD ABAS | KAZEMI TABAR S.K. | HAMIDI H. | Issue Writer Certificate

Keywords

ALFALFAQ4

MEDICAGOQ4

DROUGHTQ4

TISSUE CULTUREQ4

SOMACLONAL VARIATIONQ4

POLYETHYLENE GLYCOLQ4

Abstract

Drought stress in plants in arid and semi arid zones is a normal phenomenon. Inviter selection using TISSUE CULTURE can help to determine and evaluate the tolerant genotypes. It could be used as a complementary of a classical breeding method for producing, maintaining and applying of germplasm. In this experiment, a Yazdi seed which was selected previously using in vivo selection for DROUGHT tolerance, was used. Assessment and selection of calli in a completely randomized design (CRD) with control and different concentration of POLYETHYLENE GLYCOL (PEG) 30, 60, 90, 120 and 150 gr/lit, (10 replication in each treatment) was carried out. In the next step, the rate of regeneration and invitro selection is evaluated for DROUGHT tolerance with 40 replication in each treatment. In hydroponic experiment, plant selection for DROUGHT tolerance was done in - 12bar potential. The results of invitro selection showed that low concentration of PEG, has not significant effect on callus production. There was no significant difference on seed germination and plantlets growth, in population derived from different seeds under PEG stress.

Cites

USE OF SOMACLONAL VARIATION ON IMPROVEMENT OF DROUGHT RESISTANT LINES OF CARROT (DAUCUS CAROTA L.)

References

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APA: Copy

ASKARI, H., SAFARNEZHAD, ABAS, KAZEMI TABAR, S.K., & HAMIDI, H.. (2004). STUDY OF DROUGHT TOLERANCE IMPROVEMENT IN ALFALFA (MEDICAGO SATIVA L.) USING SOMACLONAL VARIATION. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 12(2), 117-130. SID. https://sid.ir/paper/104332/en

Vancouver: Copy

ASKARI H., SAFARNEZHAD ABAS, KAZEMI TABAR S.K., HAMIDI H.. STUDY OF DROUGHT TOLERANCE IMPROVEMENT IN ALFALFA (MEDICAGO SATIVA L.) USING SOMACLONAL VARIATION. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2004;12(2):117-130. Available from: https://sid.ir/paper/104332/en

IEEE: Copy

H. ASKARI, ABAS SAFARNEZHAD, S.K. KAZEMI TABAR, and H. HAMIDI, “STUDY OF DROUGHT TOLERANCE IMPROVEMENT IN ALFALFA (MEDICAGO SATIVA L.) USING SOMACLONAL VARIATION,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 12, no. 2, pp. 117–130, 2004, [Online]. Available: https://sid.ir/paper/104332/en

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