GENETIC DIVERSITY OF DIFFERENT POPULATIONS OF IRANIAN TEUCRIUM POLIUM L. USING RAPD MARKERS

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PESARAKLU A.; MIANABADI M.; BAGHERIEH NAJJAR M.B.; SATTARIAN A.; BAGHIZADEH A.

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Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2013 | Volume:21 | Issue:1 (41) Page(s): 24-32

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Title

GENETIC DIVERSITY OF DIFFERENT POPULATIONS OF IRANIAN TEUCRIUM POLIUM L. USING RAPD MARKERS

Author(s)

Keywords

TEUCRIUM POLIUM L.Q2

GENETIC DIVERSITYQ2

MOLECULAR MARKERSQ1

RAPDQ2

Abstract

Genetic diversity of 6 populations of TEUCRIUM POLIUM L., collected from different locations of Iran, was evaluated using random amplified polymorphic DNA (RAPD) data. Total genomic DNA was extracted from young leaves of plants and polymerase chain reaction (PCR) was performed using 8 RAPD primers. Sixty eight bands were obtained of which, 61 bands (89.71%) were polymorphic. All amplified fragments in each sample were scored based on presence (1) or absence (0). Matrix of genetic similarity was calculated by Jaccard's similarity coefficient. Cluster analysis was carried out according to UPGMA algorithm using NTSYS-pc software. The genetic similarity between genotypes ranged from 0.33 to 0.58. Results showed the population Ramian had maximum difference with other populations and classified in a separate group. Maximum similarity was observed between populations Garmab and Maraveh-Tappeh. Our results showed an appropriate level of GENETIC DIVERSITY among the investigated populations. Moreover, our data indicated that the RAPD method is a suitable approach for assessing GENETIC DIVERSITY of T. polium populations with high accuracy.

Cites

GENETIC DIVERSITY AND STRUCTURE OF IRANIAN TEUCRIUM (TEUCRIUM POLIUM L.) POPULATIONS ASSESSED BY ISSR MARKERS

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APA: Copy

PESARAKLU, A., MIANABADI, M., BAGHERIEH NAJJAR, M.B., SATTARIAN, A., & BAGHIZADEH, A.. (2013). GENETIC DIVERSITY OF DIFFERENT POPULATIONS OF IRANIAN TEUCRIUM POLIUM L. USING RAPD MARKERS. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 21(1 (41)), 24-32. SID. https://sid.ir/paper/104409/en

Vancouver: Copy

PESARAKLU A., MIANABADI M., BAGHERIEH NAJJAR M.B., SATTARIAN A., BAGHIZADEH A.. GENETIC DIVERSITY OF DIFFERENT POPULATIONS OF IRANIAN TEUCRIUM POLIUM L. USING RAPD MARKERS. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2013;21(1 (41)):24-32. Available from: https://sid.ir/paper/104409/en

IEEE: Copy

A. PESARAKLU, M. MIANABADI, M.B. BAGHERIEH NAJJAR, A. SATTARIAN, and A. BAGHIZADEH, “GENETIC DIVERSITY OF DIFFERENT POPULATIONS OF IRANIAN TEUCRIUM POLIUM L. USING RAPD MARKERS,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 21, no. 1 (41), pp. 24–32, 2013, [Online]. Available: https://sid.ir/paper/104409/en

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