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Information Journal Paper

Title

Identification of miRNAs and their target genes in red clover (Trifolium pretense)

Pages

  155-164

Abstract

 MicroRNAs (miRNAs) are a group of 18_22 nucleotides long noncoding small endogenous that derived from its precursor sequence and evolutionary conserved post-transcriptional regulatory RNAs, which show an enormous role in various biological and metabolic processes in both animals and plants. miRNA detection methods including microarray, qRT-PCR, northern blot and Bioinformatics methods in which the easiEST and cheapEST way to identify miRNA is Bioinformatics method. A Bioinformatics approach was used to identify potential miRNA in red clover. EST-based homology search was applied to find potential miRNA of red clover. We blasted publicly available EST sequences obtained from NCBI GenBank against previously known plant miRNAs. A total of six miRNA target genes based on their complementary sequences were also detected. target genes encoding mono dehydro ascorbate reductase 1 play an important role in maintaining balance in the cycle of reactive oxygen species ascorbate-glutathione, FES1 proteins that contain zinc finger domain binding DNA, RHF2A gene encoding E3 ubiquitin-protein ligase involved in the positive regulation of the gametogenesis progression, RNA helicase ATP-dependent is involved in the RNA repair and metabolism, Major facilitator superfamily (MFS) is a superfamily of membrane transport proteins that facilitate movement of small solutes across cell membranes in response to chemiosmotic gradients, AT-hook DNA binding motif protein.

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  • Cite

    APA: Copy

    NAGHAVI, M.R., & KARIMI, A.A.. (2019). Identification of miRNAs and their target genes in red clover (Trifolium pretense). IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 26(2 (52) ), 155-164. SID. https://sid.ir/paper/104491/en

    Vancouver: Copy

    NAGHAVI M.R., KARIMI A.A.. Identification of miRNAs and their target genes in red clover (Trifolium pretense). IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2019;26(2 (52) ):155-164. Available from: https://sid.ir/paper/104491/en

    IEEE: Copy

    M.R. NAGHAVI, and A.A. KARIMI, “Identification of miRNAs and their target genes in red clover (Trifolium pretense),” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 26, no. 2 (52) , pp. 155–164, 2019, [Online]. Available: https://sid.ir/paper/104491/en

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