PROTEOMIC ANALYSIS OF THE DROUGHT STRESS RESPONSE IN BARLEY (HORDEUM VULGARE L.) USING DIGE TECHNOLOGY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAHERI H.; BIHAMTA M.R.; HOSSEINZADEH A.H.; NAGHAVI M.R.; MARCH T.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2012 | Volume:7 | Issue:3 (30) Page(s): 259-268

Download Full-Text

Persian Verion

View:

533

Download:

Cites:

Information Journal Paper

Title

PROTEOMIC ANALYSIS OF THE DROUGHT STRESS RESPONSE IN BARLEY (HORDEUM VULGARE L.) USING DIGE TECHNOLOGY

Author(s)

Keywords

BARLEYQ1

DIGEQ2

DROUGHT STRESSQ3

MASS SPECTROMETRYQ3

TWO DIMENSIONAL ELECTROPHORESISQ2

Abstract

Drought is one of the most important constraints on the growth and productivity of BARLEY (Hordeum vulgare L.). A proteome analysis based on 2-D gel electrophoresis was performed in order to identify DROUGHT STRESS responsive proteins. Pot experiments were arranged in randomized block factorial design with two watering treatments, control and drought conditions, and three BARLEY genotypes. Plants were well-watered until the four leaf stage and then exposed to severe DROUGHT STRESS for 10 days. The overall effect of drought was highly significant (P<0.01), as determined by dry matter, fresh matter, relative water content and proline free content. To study protein expression changes in response to DROUGHT STRESS, proteome analysis was performed based on DIGE technology with the inclusion of an internal standard. About 768 spots were detected on CyDyes gels, of these 37 spots showed significantly changes as genotypes or genotype ×irrigation levels effects. Finally MASS SPECTROMETRY analyzing using MALDI-TOF-TOF led to the identification of 19 proteins. The functional of these proteins were identified as defense, energy, metabolism, cell structure, signal transduction and cell differentiation.

Cites

No record.

References

No record.

Cite

APA: Copy

TAHERI, H., BIHAMTA, M.R., HOSSEINZADEH, A.H., NAGHAVI, M.R., & MARCH, T.. (2012). PROTEOMIC ANALYSIS OF THE DROUGHT STRESS RESPONSE IN BARLEY (HORDEUM VULGARE L.) USING DIGE TECHNOLOGY. MODERN GENETICS JOURNAL (MGJ), 7(3 (30)), 259-268. SID. https://sid.ir/paper/116466/en

Vancouver: Copy

TAHERI H., BIHAMTA M.R., HOSSEINZADEH A.H., NAGHAVI M.R., MARCH T.. PROTEOMIC ANALYSIS OF THE DROUGHT STRESS RESPONSE IN BARLEY (HORDEUM VULGARE L.) USING DIGE TECHNOLOGY. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2012;7(3 (30)):259-268. Available from: https://sid.ir/paper/116466/en

IEEE: Copy

H. TAHERI, M.R. BIHAMTA, A.H. HOSSEINZADEH, M.R. NAGHAVI, and T. MARCH, “PROTEOMIC ANALYSIS OF THE DROUGHT STRESS RESPONSE IN BARLEY (HORDEUM VULGARE L.) USING DIGE TECHNOLOGY,” MODERN GENETICS JOURNAL (MGJ), vol. 7, no. 3 (30), pp. 259–268, 2012, [Online]. Available: https://sid.ir/paper/116466/en

Related Journal Papers