EFFECT OF PLANT GENOTYPE, EXPLANT AND AGROBACTERIUM STRAIN ON TRANSFORMATION EFFICIENCY IN RAPESEED (BRASSICA NAP US L.)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KAHRIZI D.; SALMANIAN ALI HATEF; ZEBARJADI A.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2007 | Volume:2 | Issue:3 Page(s): 53-62

Download Full-Text

Persian Verion

View:

925

Download:

Cites:

Information Journal Paper

Title

EFFECT OF PLANT GENOTYPE, EXPLANT AND AGROBACTERIUM STRAIN ON TRANSFORMATION EFFICIENCY IN RAPESEED (BRASSICA NAP US L.)

Author(s)

KAHRIZI D. | SALMANIAN ALI HATEF | ZEBARJADI A.R. | Issue Writer Certificate

Keywords

EXPLANTQ3

AGROBACTERIUMQ2

GENOTYPEQ2

RAPESSED (BRASSICA L.)Q2

TRANSFORMATIONQ3

Abstract

Rapeseed is one of the important oil seed crop in the world. Rapeseed has been improved for agronomical and economical traits via genetic engineering. The best gene TRANSFORMATIONs to rapeseed has been done through AGROBACTERIUM tumeifaciens (with different strains) method. In this research, the effect of rapeseed B.napuscultivar, EXPLANTs and AGROBACTERIUM tumefactions strain has been studied on the gus reporter genes TRANSFORMATION.The experimental design was factorial based on completely randomized design with four replications. The gus reporter genes was cloned in pBI121 as a plant expression vector. The pBI121 plasmid containing gus gene was transferred to rapeseed via A. tumeifaciens (LBA4404 and C58 (pGV3101) strains) mediated TRANSFORMATION method. Putative transgenic shoots generated from cotyledons and hypocotyls of 2 commercial cultivars of B. napus (pF-7045 -91 and SLM-046). The transformed plants were screened on MS medium containing 25 mgL-1 kanamycin. Presence and expression of the reporter gene in plants were confirmed by PCR technique and gus assay. Statistical analysis of data showed that there are significant different between rapeseed cultivars, EXPLANTs, A. tumeifa ciens strains and their interactions for transgenesis. The PF -7045 -91 GENOTYPE with 10.64% average was better than from SLM-046 variety with 4.5% average. Therefore "LBA4404 strain of A. tumefactions with 8.79% frequency in TRANSFORMATION was better than from C58 (pGV3101) with 6.39%.

Cites

No record.

References

No record.

Cite

APA: Copy

KAHRIZI, D., SALMANIAN, ALI HATEF, & ZEBARJADI, A.R.. (2007). EFFECT OF PLANT GENOTYPE, EXPLANT AND AGROBACTERIUM STRAIN ON TRANSFORMATION EFFICIENCY IN RAPESEED (BRASSICA NAP US L.). MODERN GENETICS JOURNAL (MGJ), 2(3), 53-62. SID. https://sid.ir/paper/116490/en

Vancouver: Copy

KAHRIZI D., SALMANIAN ALI HATEF, ZEBARJADI A.R.. EFFECT OF PLANT GENOTYPE, EXPLANT AND AGROBACTERIUM STRAIN ON TRANSFORMATION EFFICIENCY IN RAPESEED (BRASSICA NAP US L.). MODERN GENETICS JOURNAL (MGJ)[Internet]. 2007;2(3):53-62. Available from: https://sid.ir/paper/116490/en

IEEE: Copy

D. KAHRIZI, ALI HATEF SALMANIAN, and A.R. ZEBARJADI, “EFFECT OF PLANT GENOTYPE, EXPLANT AND AGROBACTERIUM STRAIN ON TRANSFORMATION EFFICIENCY IN RAPESEED (BRASSICA NAP US L.),” MODERN GENETICS JOURNAL (MGJ), vol. 2, no. 3, pp. 53–62, 2007, [Online]. Available: https://sid.ir/paper/116490/en

Related Journal Papers