AGROINFILTRATION AS A TECHNIQUE FOR RAPID ASSAYS FOR EVALUATING GENE EXPRESSION SYSTEMS IN COMMON BEAN

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MOZAFARI Z.; MIRAKHORLI N.; SHIRAN B.; KHODAMBASHI M.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2013 | Volume:8 | Issue:3 (34) Page(s): 273-282

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Title

AGROINFILTRATION AS A TECHNIQUE FOR RAPID ASSAYS FOR EVALUATING GENE EXPRESSION SYSTEMS IN COMMON BEAN

Author(s)

MOZAFARI Z. | MIRAKHORLI N. | SHIRAN B. | KHODAMBASHI M. | Issue Writer Certificate

Keywords

AGROBACTERIUMQ2

BEANQ2

GENE EXPRESSION SYSTEMQ3

TRANSIENT EXPRESSIONQ2

Abstract

Transient expression of genes using AGROBACTERIUM (Agroinfiltration) is a powerful tool for the analysis of foreign gene expression and function in different GENE EXPRESSION SYSTEMs in plant before stable plant transformation. However, the production of stable transgenic plants is a lengthy process, in transient assays, it is possible to measure gene expression within a very short time. With simple infiltration of AGROBACTERIUM cells carrying appropriate gene constructs into BEAN intact leaves, TRANSIENT EXPRESSION can be performed within 3 days without using expensive instruments or complicated procedures. In this study, We analysed different regulatory gene expression factors affecting TRANSIENT EXPRESSION following agroinfiltration in BEAN by injection suspensions of the AGROBACTERIUM carrying the plasmid constructs of gene interest. After three days the amount of soluble protein in the leaves was measured and compared. This study proposed to evaluate the best systems expression in transgenic BEAN plants, using agroinfiltration technique. The results demonstrate that between the three systems expression the highest level of protein is producted by the system that makes the transfer and accumulation of proteins in the endoplasmic reticulumin this study. The efficiency of agroinfiltration was 24-50%.

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APA: Copy

MOZAFARI, Z., MIRAKHORLI, N., SHIRAN, B., & KHODAMBASHI, M.. (2013). AGROINFILTRATION AS A TECHNIQUE FOR RAPID ASSAYS FOR EVALUATING GENE EXPRESSION SYSTEMS IN COMMON BEAN. MODERN GENETICS JOURNAL (MGJ), 8(3 (34)), 273-282. SID. https://sid.ir/paper/116553/en

Vancouver: Copy

MOZAFARI Z., MIRAKHORLI N., SHIRAN B., KHODAMBASHI M.. AGROINFILTRATION AS A TECHNIQUE FOR RAPID ASSAYS FOR EVALUATING GENE EXPRESSION SYSTEMS IN COMMON BEAN. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2013;8(3 (34)):273-282. Available from: https://sid.ir/paper/116553/en

IEEE: Copy

Z. MOZAFARI, N. MIRAKHORLI, B. SHIRAN, and M. KHODAMBASHI, “AGROINFILTRATION AS A TECHNIQUE FOR RAPID ASSAYS FOR EVALUATING GENE EXPRESSION SYSTEMS IN COMMON BEAN,” MODERN GENETICS JOURNAL (MGJ), vol. 8, no. 3 (34), pp. 273–282, 2013, [Online]. Available: https://sid.ir/paper/116553/en

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