CLONING AND ANALYSIS OF PHYSICO-CHEMICAL AND STRUCTURAL CHARACTERISTICS OF A H-TYPE THIOREDOXIN GENE, BELONGING TO SUBGROUP I FROM GRAPE (VITIS VINIFERA L. CV. ASKARI) BERRY TISSUE

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HEIDARI JAPELAGHI R.; HADDAD R.; GAROUSI GH.A.; NAVABPOUR S.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2011 | Volume:6 | Issue:2 (25) Page(s): 105-120

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Title

CLONING AND ANALYSIS OF PHYSICO-CHEMICAL AND STRUCTURAL CHARACTERISTICS OF A H-TYPE THIOREDOXIN GENE, BELONGING TO SUBGROUP I FROM GRAPE (VITIS VINIFERA L. CV. ASKARI) BERRY TISSUE

Author(s)

HEIDARI JAPELAGHI R. | HADDAD R. | GAROUSI GH.A. | NAVABPOUR S. | Issue Writer Certificate

Keywords

CLONINGQ2

GRAPEQ2

H -TYPE THIOREDOXINQ2

ISOLATIONQ2

THREE DIMENSIONAL STRUCTUREQ2

Abstract

thioredoxins (Trxs) are small ubiquitous disulfide reductases that are present in all organisms from prokaryotes to higher eukaryotes and involve in the cell redox regulation. In contrast to other organisms, plants contain six different Trx types: f, m, x, y, o and h that only h -type Trx has been the most extensively studied and consists of multiple forms that involved in different processes including cellular protection against oxidative stress. CLONING of ah -type thioredoxin gene was performed from GRAPE (Vitis vinifera L. cv. Askari) berry tissue by reverse transcription polymerase chain reaction (RT-PCR) technique, and then its physico-chemical, structural and phylogenetic characteristics was analyzed. The nucleotide sequence of open reading frame, called VvTrxh 4, was revealed a 345 bp long with a deduced amino acid of 114 residues, possessing a WCGPC active site. The estimated molecular mass and the predicted isoelectric point of the deduced polypeptide were 12.76 kDa and 5.22 respectively. Prediction of three-dimensional structure of VvTrxh 4 using the crystal structure of AtTrx h 1 from Arabidopsiswas consistent with these predictions and with five b-sheets and four α-helixes, contains the thioredoxin fold. The deduced protein sequence was shown a high similarity to thioredoxinsh isolated from plants, e.g. Populus trichocarpa (89% similar with Pth3) and Citrus cv. Shiranuhi (86% similar with Csh). Phylogenetic studies using of thioredoxin from other plants was indicated that VvTrx h 4 gene is a thioredoxinh, belonging to the subclass IA from subgroup I.

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References

IDENTIFICATION, CLONING AND CHARACTERIZATION OF A THIOREDOXIN H (VVTRXH10) GENE ISOLATED FROM GRAPE (VITIS VINIFERA L.) CV. YAQUTI BERRY TISSUE

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APA: Copy

HEIDARI JAPELAGHI, R., HADDAD, R., GAROUSI, GH.A., & NAVABPOUR, S.. (2011). CLONING AND ANALYSIS OF PHYSICO-CHEMICAL AND STRUCTURAL CHARACTERISTICS OF A H-TYPE THIOREDOXIN GENE, BELONGING TO SUBGROUP I FROM GRAPE (VITIS VINIFERA L. CV. ASKARI) BERRY TISSUE. MODERN GENETICS JOURNAL (MGJ), 6(2 (25)), 105-120. SID. https://sid.ir/paper/116598/en

Vancouver: Copy

HEIDARI JAPELAGHI R., HADDAD R., GAROUSI GH.A., NAVABPOUR S.. CLONING AND ANALYSIS OF PHYSICO-CHEMICAL AND STRUCTURAL CHARACTERISTICS OF A H-TYPE THIOREDOXIN GENE, BELONGING TO SUBGROUP I FROM GRAPE (VITIS VINIFERA L. CV. ASKARI) BERRY TISSUE. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2011;6(2 (25)):105-120. Available from: https://sid.ir/paper/116598/en

IEEE: Copy

R. HEIDARI JAPELAGHI, R. HADDAD, GH.A. GAROUSI, and S. NAVABPOUR, “CLONING AND ANALYSIS OF PHYSICO-CHEMICAL AND STRUCTURAL CHARACTERISTICS OF A H-TYPE THIOREDOXIN GENE, BELONGING TO SUBGROUP I FROM GRAPE (VITIS VINIFERA L. CV. ASKARI) BERRY TISSUE,” MODERN GENETICS JOURNAL (MGJ), vol. 6, no. 2 (25), pp. 105–120, 2011, [Online]. Available: https://sid.ir/paper/116598/en

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