THERMAL DENATURATION OF α-AMYLASE FROM BACILLUS AMYLOLIQUEFACIENS IN THE PRES-ENCE OF SODIUM DODECYL SULPHATE

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SHAREGHI B.; ARABI M.

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Paper Information

Journal: IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE Year:2008 | Volume:32 | Issue:A2 Page(s): 135-140

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Title

THERMAL DENATURATION OF α-AMYLASE FROM BACILLUS AMYLOLIQUEFACIENS IN THE PRES-ENCE OF SODIUM DODECYL SULPHATE

Author(s)

SHAREGHI B. | ARABI M. | Issue Writer Certificate

Keywords

STABILITYQ3

DENATURATIONQ3

THERMODYNAMICQ3

SDSQ3

α-AMYLQ3

Abstract

The thermal DENATURATION of a-Amylase from Bacillus amyloliquefaciens has been investigated in the presence and absence of sodium dodecyl sulphate (SDS) over the temperature range (293-373) K in 20 mM sodium phosphate buffer, pH 6.9, using temperature scanning spectroscopy. The presence of SDS caused the destabilization of a-Amylase resulting in a decrease in the temperature of unfolding with an increase in SDS concentration. The THERMODYNAMIC parameters for unfolding of a-Amylase were determined in terms of the two-state model, using the temperature dependence of the spectroscopic behaviour of the enzyme at 280 nm. The result from specific activity measurements in the presence of SDS is in conformity with the results from thermal studies.

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APA: Copy

SHAREGHI, B., & ARABI, M.. (2008). THERMAL DENATURATION OF α-AMYLASE FROM BACILLUS AMYLOLIQUEFACIENS IN THE PRES-ENCE OF SODIUM DODECYL SULPHATE. IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE, 32(A2), 135-140. SID. https://sid.ir/paper/117956/en

Vancouver: Copy

SHAREGHI B., ARABI M.. THERMAL DENATURATION OF α-AMYLASE FROM BACILLUS AMYLOLIQUEFACIENS IN THE PRES-ENCE OF SODIUM DODECYL SULPHATE. IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE[Internet]. 2008;32(A2):135-140. Available from: https://sid.ir/paper/117956/en

IEEE: Copy

B. SHAREGHI, and M. ARABI, “THERMAL DENATURATION OF α-AMYLASE FROM BACILLUS AMYLOLIQUEFACIENS IN THE PRES-ENCE OF SODIUM DODECYL SULPHATE,” IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE, vol. 32, no. A2, pp. 135–140, 2008, [Online]. Available: https://sid.ir/paper/117956/en

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