Isolation and identification of Harzianum clade species of Trichoderma from Khorramabad County

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Mirzaeipour Z; Bazgir Eidi; Zafari D; Darvishnia M

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Journal Paper

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Journal: MYCOLOGIA IRANICA Year:2023 | Volume:10 | Issue:2 Page(s): 67-78

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Information Journal Paper

Title

Isolation and identification of Harzianum clade species of Trichoderma from Khorramabad County

Author(s)

Mirzaeipour Z | Bazgir Eidi | Zafari D | Darvishnia M | Issue Writer Certificate

Keywords

Iran

Morphology

phylogeny

Taxonomy

tef1-α

Trichoderma

Abstract

The genus Trichoderma consists of globally distributed fungi. Among them, T. harzianum, is one of the most commonly collected Trichoderma species, which had been known as an aggregate species. In the present study, using a Trichoderma selective medium, 20 isolates belonging to 5 Trichoderma species of the Harzianum clad were isolated from soil samples collected from diverse geographical regions of Khorramabad County, Iran. Comparing the cultural and phenotypic criteria combined with molecular tests of the tef1 gene sequences of isolated fungi, five species namely, T. afroharzianum, T. atrobrunneum, T. guizhouense, T. harzianum and T. pholiotae were identified and their descriptions and figures are presented in this paper. T. harzianum had the highest frequency among the 5 species. T. pholiotae is reported for the first time from Iran.

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