OPTIMIZATION OF TRANSIENT EXPRESSION OF UIDA GENE IN ANDROGENIC EMBRYOS OF WHEAT (TRITICUM AESTIVUM L. CV. FALAT) VIA PARTICLE BOMBARDMENT

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GHARANJIK SH.; MOEINI A.; MOUSAVI AMIR; ALIZADEH HOUSHANG

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Journal: Iranian Journal of Biotechnology Year:2008 | Volume:6 | Issue:4 Page(s): 207-213

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Title

OPTIMIZATION OF TRANSIENT EXPRESSION OF UIDA GENE IN ANDROGENIC EMBRYOS OF WHEAT (TRITICUM AESTIVUM L. CV. FALAT) VIA PARTICLE BOMBARDMENT

Author(s)

GHARANJIK SH. | MOEINI A. | MOUSAVI AMIR | ALIZADEH HOUSHANG | Issue Writer Certificate

Keywords

HEXAPLOID WHEATQ4

TRANSIENT GENE EXPRESSIONQ2

GUSQ3

PARTICLE BOMBARDMENTQ3

ANDROGENIC EMBRYOSQ3

Abstract

Haploid microspore-derived embryos (MDEs) of wheat were obtained by in vitro androgenesis. These embryos were employed to evaluate the transient expression of GUS gene (uidA) following PARTICLE BOMBARDMENT. Using the Bio-Rad PDS-1000/He system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0.6 mm, 1 mm and 1.6 mm), DNA and microcarrier concentrations (0.5 mg of DNA with 150 mg of gold particles or 1.0 mg of DNA with 300 mg of gold particles/bombardment) and bombardment numbers (1x (single) and 2x (double)) were assessed. The effect of high osmoticum in the bombardment medium (0.3 M mannitol and 0.4 M maltose) and the age of embryos were also evaluated. Optimal expression in MDEs was obtained using the following conditions of double bombardment at 1350 psi, 9 cm target distance, a 1 mm gold particle size, 1.0 mg of DNA with 300 mg of gold particles/bombardment, and osmotic pretreatment of 4-6 weeks old embryos using 0.4 M maltose for 6 h before and 16 h after bombardment. The optimized transformation protocol presented in this study is expected to improve development of commercial transgenic wheat lines expressing desirable agronomic traits.

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APA: Copy

GHARANJIK, SH., MOEINI, A., MOUSAVI, AMIR, & ALIZADEH, HOUSHANG. (2008). OPTIMIZATION OF TRANSIENT EXPRESSION OF UIDA GENE IN ANDROGENIC EMBRYOS OF WHEAT (TRITICUM AESTIVUM L. CV. FALAT) VIA PARTICLE BOMBARDMENT. IRANIAN JOURNAL OF BIOTECHNOLOGY, 6(4), 207-213. SID. https://sid.ir/paper/139207/en

Vancouver: Copy

GHARANJIK SH., MOEINI A., MOUSAVI AMIR, ALIZADEH HOUSHANG. OPTIMIZATION OF TRANSIENT EXPRESSION OF UIDA GENE IN ANDROGENIC EMBRYOS OF WHEAT (TRITICUM AESTIVUM L. CV. FALAT) VIA PARTICLE BOMBARDMENT. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2008;6(4):207-213. Available from: https://sid.ir/paper/139207/en

IEEE: Copy

SH. GHARANJIK, A. MOEINI, AMIR MOUSAVI, and HOUSHANG ALIZADEH, “OPTIMIZATION OF TRANSIENT EXPRESSION OF UIDA GENE IN ANDROGENIC EMBRYOS OF WHEAT (TRITICUM AESTIVUM L. CV. FALAT) VIA PARTICLE BOMBARDMENT,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 6, no. 4, pp. 207–213, 2008, [Online]. Available: https://sid.ir/paper/139207/en

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