IN SILICO FUSION OF EPSILON AND BETA TOXIN GENES OF CLOSTRIDIUM PERFRINGENS TYPES D AND B

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PILEHCHIAN LANGROUDI REZA; AGHAIYPOUR KHOSROW; SHAMSARA MAHDI; GHORASHI SAIED ALI

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Journal: Iranian Journal of Biotechnology Year:2012 | Volume:10 | Issue:1 Page(s): 54-60

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Title

IN SILICO FUSION OF EPSILON AND BETA TOXIN GENES OF CLOSTRIDIUM PERFRINGENS TYPES D AND B

Author(s)

PILEHCHIAN LANGROUDI REZA | AGHAIYPOUR KHOSROW | SHAMSARA MAHDI | GHORASHI SAIED ALI | Issue Writer Certificate

Keywords

CLOSTRIDIUM PERFRINGENSQ2

EPSILON TOXINQ2

BETA TOXINQ2

FUSIONQ2

IN SILICOQ2

Abstract

Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective protein expression. Epsilon and BETA TOXINs are the most potent Clostridial toxins and cause disease in animals. This study describes IN SILICO FUSION of CLOSTRIDIUM PERFRINGENS types D and B epsilon and BETA TOXIN genes that was used for cloning in E. coli. The etx and cpb genes were retrieved from the GenBank and a FUSION gene was designed to produce a chimeric FUSION protein. Secondary and tertiary structures and specificities of FUSION protein were determined by online software. Results showed that the designed FUSION gene construction is suitable for chimeric FUSION protein expression.

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APA: Copy

PILEHCHIAN LANGROUDI, REZA, AGHAIYPOUR, KHOSROW, SHAMSARA, MAHDI, & GHORASHI, SAIED ALI. (2012). IN SILICO FUSION OF EPSILON AND BETA TOXIN GENES OF CLOSTRIDIUM PERFRINGENS TYPES D AND B. IRANIAN JOURNAL OF BIOTECHNOLOGY, 10(1), 54-60. SID. https://sid.ir/paper/139248/en

Vancouver: Copy

PILEHCHIAN LANGROUDI REZA, AGHAIYPOUR KHOSROW, SHAMSARA MAHDI, GHORASHI SAIED ALI. IN SILICO FUSION OF EPSILON AND BETA TOXIN GENES OF CLOSTRIDIUM PERFRINGENS TYPES D AND B. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2012;10(1):54-60. Available from: https://sid.ir/paper/139248/en

IEEE: Copy

REZA PILEHCHIAN LANGROUDI, KHOSROW AGHAIYPOUR, MAHDI SHAMSARA, and SAIED ALI GHORASHI, “IN SILICO FUSION OF EPSILON AND BETA TOXIN GENES OF CLOSTRIDIUM PERFRINGENS TYPES D AND B,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 1, pp. 54–60, 2012, [Online]. Available: https://sid.ir/paper/139248/en

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