مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

937
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

173
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

PROTECTIVE PROPERTIES OF NONTOXIC RECOMBINANT EXOTOXIN A (DOMAIN I-II) AGAINST PSEUDOMONAS AERUGINOSA INFECTION

Pages

  193-198

Abstract

 Background: Antibiotic resistance and the need for long-term treatments especially for chronic infections necessitate the development of a vaccine against PSEUDOMONAS AERUGINOSA infection.Objectives: In this study, recombinant EXOTOXIN A (domains I and II), (ExoA I-II) protein was expressed, purified and its immunological characteristics were evaluated in a mouse model.Materials and Methods: The genomic DNA was extracted from P. aeruginosa strain PAO1. The DNA encoding for domains I and II of EXOTOXIN A was amplified by PCR and cloned into the pET22b expression vector. The construct was then transformed into E. coli BL21 and the protein expression was evaluated by the SDS-PAGE method. The Ni-NTA affinity chromatography was used for recombinant protein purification. Mice were then immunized subcutaneously on day 0, 21, 42 and 72 with EXOTOXIN A (Domains I, II). Antibody production was evaluated by the ELISA method. The immunized and control group mice were exposed to an approximate 2 × LD50 (7.5 × 107 CFU) of clinical strain of mucoid P. aeruginosa.Results: Sequencing of the cloned gene showed that the sequence of ExoA I-II gene was in accordance with ExoA I-II from P. aeruginosa PAO1. SDS-PAGE analysis indicated the expression of recombinant protein with a molecular weight of 45 KDa. Vaccination with ExoA I-II produced a significant amount of specific IgG antibodies in mice. Also immunization of mice with ExoA I-II increased survival times against intra-peritoneal challenge with an approximate 7.5 × 107 CFU (2 × LD50) of clinical strain of P. aeruginosa.Conclusions: Results of this study suggested that recombinant ExoA I-II is a highly immunogenic protein which can be used as a new VACCINE CANDIDATE against P. aeruginosa.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    TANOMAND, ASGHAR, NAJAR PEERAYEH, SHAHIN, FARAJNIA, SAFAR, & MAJIDI, JAFAR. (2013). PROTECTIVE PROPERTIES OF NONTOXIC RECOMBINANT EXOTOXIN A (DOMAIN I-II) AGAINST PSEUDOMONAS AERUGINOSA INFECTION. IRANIAN JOURNAL OF BIOTECHNOLOGY, 11(3 (43)), 193-198. SID. https://sid.ir/paper/139260/en

    Vancouver: Copy

    TANOMAND ASGHAR, NAJAR PEERAYEH SHAHIN, FARAJNIA SAFAR, MAJIDI JAFAR. PROTECTIVE PROPERTIES OF NONTOXIC RECOMBINANT EXOTOXIN A (DOMAIN I-II) AGAINST PSEUDOMONAS AERUGINOSA INFECTION. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2013;11(3 (43)):193-198. Available from: https://sid.ir/paper/139260/en

    IEEE: Copy

    ASGHAR TANOMAND, SHAHIN NAJAR PEERAYEH, SAFAR FARAJNIA, and JAFAR MAJIDI, “PROTECTIVE PROPERTIES OF NONTOXIC RECOMBINANT EXOTOXIN A (DOMAIN I-II) AGAINST PSEUDOMONAS AERUGINOSA INFECTION,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 11, no. 3 (43), pp. 193–198, 2013, [Online]. Available: https://sid.ir/paper/139260/en

    Related Journal Papers

    Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops





    Move to top