MOLECULAR ASSESSMENT OF CLONAL FIDELITY IN MICROPROPAGATED GRAPE (VITIS SPP.) ROOTSTOCK GENOTYPES USING RAPD AND ISSR MARKERS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ALIZADEH M.; SINGH S.K.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Biotechnology Year:2009 | Volume:7 | Issue:1 Page(s): 37-44

Download Full-Text

Persian Verion

View:

1,316

Download:

373

Cites:

Information Journal Paper

Title

MOLECULAR ASSESSMENT OF CLONAL FIDELITY IN MICROPROPAGATED GRAPE (VITIS SPP.) ROOTSTOCK GENOTYPES USING RAPD AND ISSR MARKERS

Author(s)

ALIZADEH M. | SINGH S.K. | Issue Writer Certificate

Keywords

VITIS ROOTSTOCKSQ3

MICROPROPAGATIONQ2

CLONAL FIDELITYQ3

RAPDQ3

ISSRQ3

Abstract

Micropropagated plantlets derived from three different grape rootstock genotypes namely, Dogridge (Vitis champini), SO4 (V. berlandieri×V. rupestris) and ARI-H-144 (V. vinifera×V. labrusca) were subjected to randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vitro derived plantlets. A dozen RAPD (10-mer) and ten ISSR (dinucleotide contained repeats) primers were used for PCR and reproducible band profiles were obtained. The 84 and 81 distinct and scorable band classes (a total of 1,914 and 1,980 scorable bands) with an average of 7.0 and 8.1 bands per primer were obtained by RAPD and ISSR, respectively. Although higher numbers of bands were obtained by ISSR rather than RAPD, but none of the primers showed polymorphism among micropropagated plantlets and their respective mother plants. The profiles generated based on the two marker systems were found to be highly uniform and monomorphic. Cluster analysis further confirmed genetic stability of micropropagated plantlets. Jaccard’s similarity coefficients obtained for both markers in mother plants and their in vitro regenerants were estimated to be 1.00 but three sets of genotypes were grouped into two major clusters with similarity coefficients of 0.53 (RAPD) and 0.63 (ISSR). The molecular analyses precisely proved the production of genetically stable grape plantlets and certified the application of MICROPROPAGATION protocol to be developed on a commercial scale.

Cites

No record.

References

No record.

Cite

APA: Copy

ALIZADEH, M., & SINGH, S.K.. (2009). MOLECULAR ASSESSMENT OF CLONAL FIDELITY IN MICROPROPAGATED GRAPE (VITIS SPP.) ROOTSTOCK GENOTYPES USING RAPD AND ISSR MARKERS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 7(1), 37-44. SID. https://sid.ir/paper/139272/en

Vancouver: Copy

ALIZADEH M., SINGH S.K.. MOLECULAR ASSESSMENT OF CLONAL FIDELITY IN MICROPROPAGATED GRAPE (VITIS SPP.) ROOTSTOCK GENOTYPES USING RAPD AND ISSR MARKERS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2009;7(1):37-44. Available from: https://sid.ir/paper/139272/en

IEEE: Copy

M. ALIZADEH, and S.K. SINGH, “MOLECULAR ASSESSMENT OF CLONAL FIDELITY IN MICROPROPAGATED GRAPE (VITIS SPP.) ROOTSTOCK GENOTYPES USING RAPD AND ISSR MARKERS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 7, no. 1, pp. 37–44, 2009, [Online]. Available: https://sid.ir/paper/139272/en

Related Journal Papers