EXPRESSION PATTERN OF THE SYNTHETIC PATHOGEN-INDUCIBLE PROMOTER (SYNP-FF) IN THE TRANSGENIC CANOLA IN RESPONSE TO SCLEROTINIA SCLEROTIORUM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHOKOUHIFAR F.; ZAMANI M.R.; MOTALEBI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Biotechnology Year:2011 | Volume:9 | Issue:1 Page(s): 1-10

Download Full-Text

Persian Verion

View:

1,102

Download:

225

Cites:

Information Journal Paper

Title

EXPRESSION PATTERN OF THE SYNTHETIC PATHOGEN-INDUCIBLE PROMOTER (SYNP-FF) IN THE TRANSGENIC CANOLA IN RESPONSE TO SCLEROTINIA SCLEROTIORUM

Author(s)

SHOKOUHIFAR F. | ZAMANI M.R. | MOTALEBI M. | Issue Writer Certificate

Keywords

CIS-ACTING ELEMENTQ3

SYNTHETIC PROMOTERQ3

PATHOGEN-INDUCIBLEQ3

BRASSICA NAPUSQ2

FUNGAL ELICITORQ3

REPORTER GENEQ2

SCLEROTINIA SCLEROTIORUMQ3

Abstract

Sclerotinia sclerotiorum is a phytopathogenic fungus which causes serious yield losses in canola. A pathogen inducible-promoter can facilitate the production of Sclerotinia-resistant transgenic canola plants. In this study, the “gain of function approach” was adopted for the construction of a PATHOGEN-INDUCIBLE promoter. The SYNTHETIC PROMOTER technique was used, which involved the insertion of the dimerized form of the cisacting element (F) upstream of the minimal CaMV 35S promoter, which drives the expression of the β-glucronidase (GUS) gene. The pGFF construct containing this SYNTHETIC PROMOTER (SynP-FF) was used for stable transformation of the canola plant. Fluorometric GUS expression analysis indicated that the SynP-FF promoter is responsive to methyl jasmonate and S. sclerotiorum treatments. The results of histochemical GUS expression patterns showed strong reporter expression in leaf flower and stem tissues of canola. Hence, the SynP-FF SYNTHETIC PROMOTER, carrying fungal PATHOGEN-INDUCIBLE features, could be considered as a valuable tool for controlling the expression of transgenes to improve resistance against the same lifestyle pathogens.

Cites

No record.

References

No record.

Cite

APA: Copy

SHOKOUHIFAR, F., ZAMANI, M.R., & MOTALEBI, M.. (2011). EXPRESSION PATTERN OF THE SYNTHETIC PATHOGEN-INDUCIBLE PROMOTER (SYNP-FF) IN THE TRANSGENIC CANOLA IN RESPONSE TO SCLEROTINIA SCLEROTIORUM. IRANIAN JOURNAL OF BIOTECHNOLOGY, 9(1), 1-10. SID. https://sid.ir/paper/139281/en

Vancouver: Copy

SHOKOUHIFAR F., ZAMANI M.R., MOTALEBI M.. EXPRESSION PATTERN OF THE SYNTHETIC PATHOGEN-INDUCIBLE PROMOTER (SYNP-FF) IN THE TRANSGENIC CANOLA IN RESPONSE TO SCLEROTINIA SCLEROTIORUM. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2011;9(1):1-10. Available from: https://sid.ir/paper/139281/en

IEEE: Copy

F. SHOKOUHIFAR, M.R. ZAMANI, and M. MOTALEBI, “EXPRESSION PATTERN OF THE SYNTHETIC PATHOGEN-INDUCIBLE PROMOTER (SYNP-FF) IN THE TRANSGENIC CANOLA IN RESPONSE TO SCLEROTINIA SCLEROTIORUM,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 9, no. 1, pp. 1–10, 2011, [Online]. Available: https://sid.ir/paper/139281/en

Related Journal Papers