PRODUCTION OF RECOMBINANT HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR BY PICHIA PASTORIS

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BAHRAMI A.; SHOJA ALSADATI S.A.; KHALILZADEH RASOUL; SAEIDI NIA A.R.; VASHEGHANI FARAAHANI E.; MOHAMMADIAN MOUSA ABADI J.

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Journal: Iranian Journal of Biotechnology Year:2007 | Volume:5 | Issue:3 Page(s): 162-169

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Title

PRODUCTION OF RECOMBINANT HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR BY PICHIA PASTORIS

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Keywords

PICHIA PASTORISQ3

RHG-CSFQ3

FED-BATCH CULTUREQ3

METHANOLQ3

Abstract

Human granulocyte-colony stimulating factor (hGCSF) cDNA was expressed in the methylotrophic yeast PICHIA PASTORIS under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using pPIC9 vector.Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as, temperature and pH on the P. pastoris cell growth and hGCSF production during fermentation were investigated.Cell growth and hG-CSF production were found to be optimal at 28°C and pH 6.0. A fed-batch fermentation process was also developed to obtain high cell density and higher levels of protein expression. Using a high cell density cultivation method, cell dry weight and hGCSF concentration reached 100 g/l and 35 mg/l, respectively.

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APA: Copy

BAHRAMI, A., SHOJA ALSADATI, S.A., KHALILZADEH, RASOUL, SAEIDI NIA, A.R., VASHEGHANI FARAAHANI, E., & MOHAMMADIAN MOUSA ABADI, J.. (2007). PRODUCTION OF RECOMBINANT HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR BY PICHIA PASTORIS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 5(3), 162-169. SID. https://sid.ir/paper/139296/en

Vancouver: Copy

BAHRAMI A., SHOJA ALSADATI S.A., KHALILZADEH RASOUL, SAEIDI NIA A.R., VASHEGHANI FARAAHANI E., MOHAMMADIAN MOUSA ABADI J.. PRODUCTION OF RECOMBINANT HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR BY PICHIA PASTORIS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2007;5(3):162-169. Available from: https://sid.ir/paper/139296/en

IEEE: Copy

A. BAHRAMI, S.A. SHOJA ALSADATI, RASOUL KHALILZADEH, A.R. SAEIDI NIA, E. VASHEGHANI FARAAHANI, and J. MOHAMMADIAN MOUSA ABADI, “PRODUCTION OF RECOMBINANT HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR BY PICHIA PASTORIS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 5, no. 3, pp. 162–169, 2007, [Online]. Available: https://sid.ir/paper/139296/en

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