CLONING OF LUXAB REPORTER GENE INTO IRANIAN PLANT PATHOGEN BACTERIAL ISOLATES PSEUDOMONAS SYRINGAE AND RALOSTONIA SOLANACERUM

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MOSTOFI S.; MASHREGHI M.; BAHREINI M.; OROOJALIAN F.; PORDELI P.

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ISSN: 2588-4824

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Journal: Iranian Plant Protection Research Year:2012 | Volume:25 | Issue:4 Page(s): 426-434

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Title

CLONING OF LUXAB REPORTER GENE INTO IRANIAN PLANT PATHOGEN BACTERIAL ISOLATES PSEUDOMONAS SYRINGAE AND RALOSTONIA SOLANACERUM

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Keywords

PSEUDOMONAS SYRINGAEQ2

RALOSTONIA SOLANACERUMQ2

LUXAB GENEQ2

STRAIN MONITORINGQ2

Abstract

The ability to monitor particular microorganism in an environment is a difficult task. Many genes permit the differentiation of strains by conferring production of unique phenotypes such as bioluminescence [marine bacterial luciferase (luxAB)]. Therefore, lux-AB gene was cloned into two endemic plant pathogens PSEUDOMONAS SYRINGAE and Ralostonia solanacearum by electrotransformation. lux-marking of above strains was carried out using miniTn-5 luxAB transposon. Purified strains were transformed with plasmid pUT containing LUXAB GENE by electroporator. Electroporation was performed in voltage 2.5 KV for 5 milisecend. All luxAB marked strains could grow on KB agar medium containing 12.5 mg/ml tetracycline and their luminescence intensity was measured by luminometer. lux-marked P. syringae and R. solanacearum were stable genetically engineered strains making them quite appropriate.

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APA: Copy

MOSTOFI, S., MASHREGHI, M., BAHREINI, M., OROOJALIAN, F., & PORDELI, P.. (2012). CLONING OF LUXAB REPORTER GENE INTO IRANIAN PLANT PATHOGEN BACTERIAL ISOLATES PSEUDOMONAS SYRINGAE AND RALOSTONIA SOLANACERUM. IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION), 25(4), 426-434. SID. https://sid.ir/paper/142660/en

Vancouver: Copy

MOSTOFI S., MASHREGHI M., BAHREINI M., OROOJALIAN F., PORDELI P.. CLONING OF LUXAB REPORTER GENE INTO IRANIAN PLANT PATHOGEN BACTERIAL ISOLATES PSEUDOMONAS SYRINGAE AND RALOSTONIA SOLANACERUM. IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION)[Internet]. 2012;25(4):426-434. Available from: https://sid.ir/paper/142660/en

IEEE: Copy

S. MOSTOFI, M. MASHREGHI, M. BAHREINI, F. OROOJALIAN, and P. PORDELI, “CLONING OF LUXAB REPORTER GENE INTO IRANIAN PLANT PATHOGEN BACTERIAL ISOLATES PSEUDOMONAS SYRINGAE AND RALOSTONIA SOLANACERUM,” IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION), vol. 25, no. 4, pp. 426–434, 2012, [Online]. Available: https://sid.ir/paper/142660/en

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