A SURVEY ON PROTEASES AND A-AMYLASE OF THE OOCYTE AND EGG OF THE PISTACHIO GREEN STINK BUG, BRACHYNEMA GERMARI KOL. (HEMIPTERA: PENTATOMIDAE)

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BIGHAM M.; HOSSEININAVEH V.; TALEBI JAHROMI K.; HOSSEININAVEH F.

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Journal: Iranian Plant Protection Research Year:2010 | Volume:24 | Issue:1 Page(s): 87-98

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Title

A SURVEY ON PROTEASES AND A-AMYLASE OF THE OOCYTE AND EGG OF THE PISTACHIO GREEN STINK BUG, BRACHYNEMA GERMARI KOL. (HEMIPTERA: PENTATOMIDAE)

Author(s)

BIGHAM M. | HOSSEININAVEH V. | TALEBI JAHROMI K. | HOSSEININAVEH F. | Issue Writer Certificate

Keywords

PISTACHIOQ3

BRACHYNEMA GERMARIQ3

CYSTEINE PROTEINASESQ3

CATHEPSIN LQ3

CATHEPSIN BQ1

α-AMYLASEQ1

Abstract

The PISTACHIO green stink bug (Brachynema germari kol.) is one of the most important pests of PISTACHIO in Iran which causes considerable damage to this valuable crop annually. For controlling insect pests such as B. germari, their physiological process have been considered to be interfered. Using common conventional substerate; hemoglobin, Maximum total proteolytic activity was measured at pH 3 suggesting the presence of CYSTEINE PROTEINASES in the egg and oocyte extract. The specific substrates for cysteine proteases, Z-Arg-ArgpNA, Z-Phe-Arg-pNA, were hydrolyzed by egg and oocyte extract strongly suggesting the presence of the CYSTEINE PROTEINASES CATHEPSIN B and CATHEPSIN L, respectively. Hydrolyzing activity of the CATHEPSIN B and L in egg extract against the substrates Z-Phe-Arg-pNA and Z-Arg-Arg-pNA was inhibited by the specific inhibitor E-64 up to 99.85% and 82.14% and in oocyte extract 63/24 and 74/62 respectively. The presence of cysteine proteases was supported by enhancement of the egg proteinase activity in the present of 1mM DTT and oocyte proteinase activity in the present of 1mM L-cysteine for Z-Phe-Arg-pNA and for Z-Arg-Arg-pNA, 99.41, 78.57 and 188.23, 27.71 respectively. In general, the results revealed the presence of cothepsin L and B proteinases in egg and oocyte extract. Alsoα- amylase presence in egg and oocyte of green stink bug was determined and the enzyme optimum pH was 6.

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APA: Copy

BIGHAM, M., HOSSEININAVEH, V., TALEBI JAHROMI, K., & HOSSEININAVEH, F.. (2010). A SURVEY ON PROTEASES AND A-AMYLASE OF THE OOCYTE AND EGG OF THE PISTACHIO GREEN STINK BUG, BRACHYNEMA GERMARI KOL. (HEMIPTERA: PENTATOMIDAE). IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION), 24(1), 87-98. SID. https://sid.ir/paper/142708/en

Vancouver: Copy

BIGHAM M., HOSSEININAVEH V., TALEBI JAHROMI K., HOSSEININAVEH F.. A SURVEY ON PROTEASES AND A-AMYLASE OF THE OOCYTE AND EGG OF THE PISTACHIO GREEN STINK BUG, BRACHYNEMA GERMARI KOL. (HEMIPTERA: PENTATOMIDAE). IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION)[Internet]. 2010;24(1):87-98. Available from: https://sid.ir/paper/142708/en

IEEE: Copy

M. BIGHAM, V. HOSSEININAVEH, K. TALEBI JAHROMI, and F. HOSSEININAVEH, “A SURVEY ON PROTEASES AND A-AMYLASE OF THE OOCYTE AND EGG OF THE PISTACHIO GREEN STINK BUG, BRACHYNEMA GERMARI KOL. (HEMIPTERA: PENTATOMIDAE),” IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION), vol. 24, no. 1, pp. 87–98, 2010, [Online]. Available: https://sid.ir/paper/142708/en

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