BRIEF REPORT: MOLECULAR DIAGNOSIS OF SPIROPLASMA CITRI IN CITRUS CULTIVARS, USING PCR-BASED ASSAY IN COMPARE WITH DAS-ELISA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOSLEMKHANI C.; SHAHBAZI R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Plant Protection Research Year:2013 | Volume:27 | Issue:1 Page(s): 139-141

Download Full-Text

Persian Verion

View:

933

Download:

Cites:

Information Journal Paper

Title

BRIEF REPORT: MOLECULAR DIAGNOSIS OF SPIROPLASMA CITRI IN CITRUS CULTIVARS, USING PCR-BASED ASSAY IN COMPARE WITH DAS-ELISA

Author(s)

MOSLEMKHANI C. | SHAHBAZI R. | Issue Writer Certificate

Keywords

SPIROPLASMA CITRIQ2

DETECTIONQ2

PCRQ3

ELISAQ2

CITRUSQ3

Abstract

SPIROPLASMA CITRI, the causal agent of stubborn disease has become one of the destructive pathogen of CITRUS industry in Iran. One of the most important strategies for control of stubborn disease is using S. citri-free mother trees, propagative material and CITRUS nurseries and disease avoidance in new orchards. In this way, the efficiency of the standardized ELISA with poly clonal antibody and PCR protocol with three different primer pairs was assessed. The results revealed that ELISA was not able to detect low titre of infection (in symptomless samples) so using ELISA techniques in certification system was not recommended and has significant problem such as false negative reaction. PCR method and specially P89f/r primers in compare with two other primers P58-1f/5r and P58-6f/4r was able to detect low titre of north and south strains of pathogen in moderate seasons. Out of a total 350 samples screened for presence of S. citri by the PCR method only 4.6 % showing positive results in symptomless samples.

Cites

No record.

References

No record.

Cite

APA: Copy

MOSLEMKHANI, C., & SHAHBAZI, R.. (2013). BRIEF REPORT: MOLECULAR DIAGNOSIS OF SPIROPLASMA CITRI IN CITRUS CULTIVARS, USING PCR-BASED ASSAY IN COMPARE WITH DAS-ELISA. IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION), 27(1), 139-141. SID. https://sid.ir/paper/142759/en

Vancouver: Copy

MOSLEMKHANI C., SHAHBAZI R.. BRIEF REPORT: MOLECULAR DIAGNOSIS OF SPIROPLASMA CITRI IN CITRUS CULTIVARS, USING PCR-BASED ASSAY IN COMPARE WITH DAS-ELISA. IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION)[Internet]. 2013;27(1):139-141. Available from: https://sid.ir/paper/142759/en

IEEE: Copy

C. MOSLEMKHANI, and R. SHAHBAZI, “BRIEF REPORT: MOLECULAR DIAGNOSIS OF SPIROPLASMA CITRI IN CITRUS CULTIVARS, USING PCR-BASED ASSAY IN COMPARE WITH DAS-ELISA,” IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION), vol. 27, no. 1, pp. 139–141, 2013, [Online]. Available: https://sid.ir/paper/142759/en

Related Journal Papers