MOLECULAR CHARACTERIZATION OF ASTER YELLOWS PHYTOPLASMA ASSOCIATED WITH CITRUS VARIETIES, USING MULTIPLEX PCR

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MOSLEMKHANI COBRA; SHAHBAZI RAHELEH; BAGHAEE RAVARI SAREH; KHELGHATIBANA FATEMEH

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Journal: JOURNAL OF CROP PROTECTION Year:2015 | Volume:4 | Issue:1 Page(s): 19-27

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Information Journal Paper

Title

MOLECULAR CHARACTERIZATION OF ASTER YELLOWS PHYTOPLASMA ASSOCIATED WITH CITRUS VARIETIES, USING MULTIPLEX PCR

Author(s)

MOSLEMKHANI COBRA | SHAHBAZI RAHELEH | BAGHAEE RAVARI SAREH | KHELGHATIBANA FATEMEH | Issue Writer Certificate

Keywords

VASCULAR LIMITEDQ2

CITRUSQ2

PHYTOPLASMAQ2

MULTIPLEX PCRQ2

Abstract

A survey was carried out on CITRUS nurseries in Mazandaran and Kerman provinces as the major CITRUS growing regions of Iran. Different varieties of orange and tangerine with abnormal symptoms were examined for the occurrence of Candidatus Liberobacter asiaticus, Spiroplasma citri and PHYTOPLASMA as the most important VASCULAR LIMITED pathogens in CITRUS. By visual inspection and molecular examination of 360 samples for the presence of fastidious prokaryotes only an occasional occurrence of PHYTOPLASMA and Spiroplasma citri were detected, using PHYTOPLASMA universal primer pairs P1/P7 and fU5/rU3 (in single tube nested PCR assay) and Spiroplasma specific primers P89-r/f and P58-6f/4r. PCR products of PHYTOPLASMAs were digested with MseI, AluI, BamHI and TaqI restriction enzymes and the digestion results showed PHYTOPLASMA related to aster yellows PHYTOPLASMA group. No amplicon was obtained for Candidatus Liberobacter asiaticus. The findings suggest when pathogen’s titre is low; symptoms of VASCULAR LIMITED disease are almost confused with disorder and deficiencies signs. So for sanitary selection and certification program, using sensitive detection method is recommended. MULTIPLEX PCR assay used in this study successfully detected Candidatus Liberibacter asiaticus, Spiroplasma citri and PHYTOPLASMA in one reaction and suggests for health certification programs.

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APA: Copy

MOSLEMKHANI, COBRA, SHAHBAZI, RAHELEH, BAGHAEE RAVARI, SAREH, & KHELGHATIBANA, FATEMEH. (2015). MOLECULAR CHARACTERIZATION OF ASTER YELLOWS PHYTOPLASMA ASSOCIATED WITH CITRUS VARIETIES, USING MULTIPLEX PCR. JOURNAL OF CROP PROTECTION, 4(1), 19-27. SID. https://sid.ir/paper/238953/en

Vancouver: Copy

MOSLEMKHANI COBRA, SHAHBAZI RAHELEH, BAGHAEE RAVARI SAREH, KHELGHATIBANA FATEMEH. MOLECULAR CHARACTERIZATION OF ASTER YELLOWS PHYTOPLASMA ASSOCIATED WITH CITRUS VARIETIES, USING MULTIPLEX PCR. JOURNAL OF CROP PROTECTION[Internet]. 2015;4(1):19-27. Available from: https://sid.ir/paper/238953/en

IEEE: Copy

COBRA MOSLEMKHANI, RAHELEH SHAHBAZI, SAREH BAGHAEE RAVARI, and FATEMEH KHELGHATIBANA, “MOLECULAR CHARACTERIZATION OF ASTER YELLOWS PHYTOPLASMA ASSOCIATED WITH CITRUS VARIETIES, USING MULTIPLEX PCR,” JOURNAL OF CROP PROTECTION, vol. 4, no. 1, pp. 19–27, 2015, [Online]. Available: https://sid.ir/paper/238953/en

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