GENETIC VARIATION OF UNCINULA NECATOR (SCHW.) BURR. CAUSAL AGENT OF GRAPE POWDERY MILDEW BY USING RAPD MOLECULAR MARKERS IN KHORASSAN PROVINCE

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HAJIAN SHAHRI M.; ZAD JAVAD; SHARIFI TEHRANI A.; OKHOVAT M.; SAFARNEZHAD ABAS

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Journal: Iranian Plant Protection Research Year:2008 | Volume:22 | Issue:1 Page(s): 49-60

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Title

GENETIC VARIATION OF UNCINULA NECATOR (SCHW.) BURR. CAUSAL AGENT OF GRAPE POWDERY MILDEW BY USING RAPD MOLECULAR MARKERS IN KHORASSAN PROVINCE

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Keywords

POWDERY MILDEWQ3

GRAPEQ2

GENETIC VARIATIONQ2

KHORASSANQ3

RAPD-PCRQ3

Abstract

Thirty-four isolates of U. necator were taken from diseased GRAPEs from different vineyards in KHORASSAN province. Sample was collected in June, July and August 2003 from the same plants. Young plant leaves reproduced through micro propagation method were used to obtain single spore isolates. A minimum of 10 leaves were used to produce fungi biomass. Amount of fungi biomass production 12 days after inoculation were changed in a range of 6 to 38 mg depending on concerned isolates. DNA from these isolates were extracted and GENETIC VARIATION among all isolates were assessed with 22 primers by using RAPD technique. Cluster analysis based on the 126 RAPD fragments obtained using 22 primers revealed two very distinct groups, one group containing 11 isolates from the first sampling, the second group containing all the remaining isolates. Isolates clustered in the first group displayed 47 RAPD fragments specific to them. These isolates did not exhibit 79 RAPD fragments present in all other isolates. Molecular data suggested that isolates clustering in two groups represent two different biotypes of U. necator, which are likely to be genetically isolated.

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References

INVESTIGATION ON THE ROLE OF CLEISTOTHECIUM IN THE OVERWINTERING OF UNCINULA NECATOR (SCHW.)BURR.CAUSAL AGENT OF GRAPE POWDERY MILDEW DISEASE IN KHORASAN PROVINCE

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APA: Copy

HAJIAN SHAHRI, M., ZAD, JAVAD, SHARIFI TEHRANI, A., OKHOVAT, M., & SAFARNEZHAD, ABAS. (2008). GENETIC VARIATION OF UNCINULA NECATOR (SCHW.) BURR. CAUSAL AGENT OF GRAPE POWDERY MILDEW BY USING RAPD MOLECULAR MARKERS IN KHORASSAN PROVINCE. IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION), 22(1), 49-60. SID. https://sid.ir/paper/142794/en

Vancouver: Copy

HAJIAN SHAHRI M., ZAD JAVAD, SHARIFI TEHRANI A., OKHOVAT M., SAFARNEZHAD ABAS. GENETIC VARIATION OF UNCINULA NECATOR (SCHW.) BURR. CAUSAL AGENT OF GRAPE POWDERY MILDEW BY USING RAPD MOLECULAR MARKERS IN KHORASSAN PROVINCE. IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION)[Internet]. 2008;22(1):49-60. Available from: https://sid.ir/paper/142794/en

IEEE: Copy

M. HAJIAN SHAHRI, JAVAD ZAD, A. SHARIFI TEHRANI, M. OKHOVAT, and ABAS SAFARNEZHAD, “GENETIC VARIATION OF UNCINULA NECATOR (SCHW.) BURR. CAUSAL AGENT OF GRAPE POWDERY MILDEW BY USING RAPD MOLECULAR MARKERS IN KHORASSAN PROVINCE,” IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION), vol. 22, no. 1, pp. 49–60, 2008, [Online]. Available: https://sid.ir/paper/142794/en

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