Comparing Applying Different Chromatographic Columns for Acrylamide Analysis in Potato Chips by High-Performance Liquid Chromatography

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SAMANIPOUR BABAK; JALILI MARYAM; REZAEI KARAMATOLLAH; SHOKROLAHI FATANEH

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Journal: Iranian Journal of Biosystems Engineering Year:2019 | Volume:50 | Issue:1 Page(s): 223-239

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Title

Comparing Applying Different Chromatographic Columns for Acrylamide Analysis in Potato Chips by High-Performance Liquid Chromatography

Author(s)

SAMANIPOUR BABAK | JALILI MARYAM | REZAEI KARAMATOLLAH | SHOKROLAHI FATANEH | Issue Writer Certificate

Keywords

AcrylamideQ1

AnalysisQ1

Chromatography ColumnQ1

Liquid ChromatographyQ1

Photodiode Array DetectorQ1

Abstract

Acrylamide is a toxic compound produced in some processed foods. The purpose of this research was to provide an easy and reliable method for measuring Acrylamide in potato chips by HPLC-PDA. For this purpose, the effect of 5 columns ODS-H-C18 Optimal, Sphere-Image 80-5 ODS 2, Zorbax Hilic Plus, Lichrosorb-100 NH2 and Shodex NH2P-50 4E were investigated on the separation and measurement of Acrylamide in chips. The best results were obtained with the use of the new generation of Shodex NH2P-50 4E amino columns, and Acrylamide peak was isolated successfully without the need for solid state purification steps. The standard calibration curve was linear in the range of 100-5000 μ g / L (R2 = 0. 997). Recycling percentages in the range of 95. 9% to 102. 2%, the detection limit and the limit of value were 9 and 27 μ g / Kg respectively. The repeatability of this method was appropriate and the relative standard deviation was 4. 39%.

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APA: Copy

SAMANIPOUR, BABAK, JALILI, MARYAM, REZAEI, KARAMATOLLAH, & SHOKROLAHI, FATANEH. (2019). Comparing Applying Different Chromatographic Columns for Acrylamide Analysis in Potato Chips by High-Performance Liquid Chromatography. IRANIAN JOURNAL OF BIOSYSTEMS ENGINEERING (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 50(1 ), 223-239. SID. https://sid.ir/paper/144542/en

Vancouver: Copy

SAMANIPOUR BABAK, JALILI MARYAM, REZAEI KARAMATOLLAH, SHOKROLAHI FATANEH. Comparing Applying Different Chromatographic Columns for Acrylamide Analysis in Potato Chips by High-Performance Liquid Chromatography. IRANIAN JOURNAL OF BIOSYSTEMS ENGINEERING (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2019;50(1 ):223-239. Available from: https://sid.ir/paper/144542/en

IEEE: Copy

BABAK SAMANIPOUR, MARYAM JALILI, KARAMATOLLAH REZAEI, and FATANEH SHOKROLAHI, “Comparing Applying Different Chromatographic Columns for Acrylamide Analysis in Potato Chips by High-Performance Liquid Chromatography,” IRANIAN JOURNAL OF BIOSYSTEMS ENGINEERING (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 50, no. 1 , pp. 223–239, 2019, [Online]. Available: https://sid.ir/paper/144542/en

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