EXTRACTION OF CODEINE FROM HUMAN URINE USING LIQUID-LIQUID-EXTRACTION METHOD AND ITS QUANTITATIVE MEASUREMENT BY GAS CHROMATOGRAPHY

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HOSSEINI TAGHAVI A.; NAZERI A.; SABZEVARI O.; AFSHAR M.; FEKRI M.

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Journal: Research in Medicine Year:2003 | Volume:26 | Issue:4 Page(s): 287-290

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Title

EXTRACTION OF CODEINE FROM HUMAN URINE USING LIQUID-LIQUID-EXTRACTION METHOD AND ITS QUANTITATIVE MEASUREMENT BY GAS CHROMATOGRAPHY

Author(s)

Keywords

CODEINEQ4

MORPHINEQ3

LIQUID-LIQUID EXTRACTIONQ4

GAS CHROMATOGRAPHYQ4

Abstract

Background: There are various methods for CODEINE extraction and detection, for this, Liquid-Liquid-Extraction (LLE) and GAS CHROMATOGRAPHY are useful tools. In case of higher recovery rate, more accurate results would be expected. In the present study, we have surveyed methods of CODEINE (and MORPHINE) extraction and detection with higher sensitivity and accuracy.Materials and methods: The urine samples were collected from opioid abusers and each sample was divided in two; one was hydrolyzed by bath boiling water for 60 minutes with HCl, whereas the other didn’t. All samples extracted by the LLE system (Chloroform/2-Propanol, 9:1, 20 min shaking and centrifugation) and quantified using a Varian GAS CHROMATOGRAPHY. For deriving, the product was exposed to BSTFA at temperature of 75 degrees centigrade for 25 min. Results: Results have revealed that the area under curve (ADC) on hydrolyzed samples was higher than non-hydrolyzed ones. The recovery rate was 89%.Conclusion: LLE can be used for CODEINE extraction since it demonstrates good sensitivity and accuracy.

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APA: Copy

HOSSEINI TAGHAVI, A., NAZERI, A., SABZEVARI, O., AFSHAR, M., & FEKRI, M.. (2003). EXTRACTION OF CODEINE FROM HUMAN URINE USING LIQUID-LIQUID-EXTRACTION METHOD AND ITS QUANTITATIVE MEASUREMENT BY GAS CHROMATOGRAPHY. PAJOUHESH DAR PEZESHKI, 26(4), 287-290. SID. https://sid.ir/paper/41633/en

Vancouver: Copy

HOSSEINI TAGHAVI A., NAZERI A., SABZEVARI O., AFSHAR M., FEKRI M.. EXTRACTION OF CODEINE FROM HUMAN URINE USING LIQUID-LIQUID-EXTRACTION METHOD AND ITS QUANTITATIVE MEASUREMENT BY GAS CHROMATOGRAPHY. PAJOUHESH DAR PEZESHKI[Internet]. 2003;26(4):287-290. Available from: https://sid.ir/paper/41633/en

IEEE: Copy

A. HOSSEINI TAGHAVI, A. NAZERI, O. SABZEVARI, M. AFSHAR, and M. FEKRI, “EXTRACTION OF CODEINE FROM HUMAN URINE USING LIQUID-LIQUID-EXTRACTION METHOD AND ITS QUANTITATIVE MEASUREMENT BY GAS CHROMATOGRAPHY,” PAJOUHESH DAR PEZESHKI, vol. 26, no. 4, pp. 287–290, 2003, [Online]. Available: https://sid.ir/paper/41633/en

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