INTRODUCTION OF COMMERCIAL PROTOCOL FOR IN VITRO PROLIFERATION OF BRASSICA OLERACEA VAR. ACEPHALA

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TAGHIZADEH MINA; SOLGI MOUSA

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Journal: Iranian Journal of Horticultural Science Year:2015 | Volume:45 | Issue:4 Page(s): 475-484

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Title

INTRODUCTION OF COMMERCIAL PROTOCOL FOR IN VITRO PROLIFERATION OF BRASSICA OLERACEA VAR. ACEPHALA

Author(s)

TAGHIZADEH MINA | SOLGI MOUSA | Issue Writer Certificate

Keywords

IN VITRO REGENERATIONQ2

KALE ORNAMENTALQ2

PLANT GROWTH REGULATORSQ2

Abstract

In recent years, ornamental kale is commonly used in landscapes due to its colorful shoots and resistance to the diverse environmental conditions. Thus, in vitro proliferation was studied in series of experiments. Different concentrations of BA (0.10.1, 0.5 and 1 mg/L), 2,4-D (0.1, 0.5 mg/L) and silver nitrate (2, 4 mg/L) were employed for the establishment stage. Treatments of the proliferation stage also included BA (0.1 and 0.5 mg/L) and IBA (0.1 and 0.5 mg/L). Acclimatization of in vitro plantlets was conducted using different concentrations of MS (complete and half strength) and vermicompost extract (direct and diluted). Results revealed that, using of 0.1 mg/L BA with one mg/L 2,4-D was able to provide regeneration in this ornamental plant. Proliferation medium supplemented with 0.5 mg/L BA caused shoot development. Finally, in vitro plantlets were successfully adapted by transferring to media containing reduced MS medium without PLANT GROWTH REGULATORS. In conclusion, by using the relatively low cost, accessible media and the least concentrations of PLANT GROWTH REGULATORS, successful regeneration of ornamental kale was obtained.

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APA: Copy

TAGHIZADEH, MINA, & SOLGI, MOUSA. (2015). INTRODUCTION OF COMMERCIAL PROTOCOL FOR IN VITRO PROLIFERATION OF BRASSICA OLERACEA VAR. ACEPHALA. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 45(4), 475-484. SID. https://sid.ir/paper/148083/en

Vancouver: Copy

TAGHIZADEH MINA, SOLGI MOUSA. INTRODUCTION OF COMMERCIAL PROTOCOL FOR IN VITRO PROLIFERATION OF BRASSICA OLERACEA VAR. ACEPHALA. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2015;45(4):475-484. Available from: https://sid.ir/paper/148083/en

IEEE: Copy

MINA TAGHIZADEH, and MOUSA SOLGI, “INTRODUCTION OF COMMERCIAL PROTOCOL FOR IN VITRO PROLIFERATION OF BRASSICA OLERACEA VAR. ACEPHALA,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 45, no. 4, pp. 475–484, 2015, [Online]. Available: https://sid.ir/paper/148083/en

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