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Information Journal Paper

Title

DIAGNOSIS OF MAREK’S DISEASE VIRUS (MDV) BY PCR

Pages

  35-39

Abstract

 Marek’s disease (MD) is a viral disease caused by the penetration and proliferation of lymphoid cells in parietal nerves and some other organs of birds and in fact as is considered, one of the ordinary and important infectious diseases in poultry. MD viruses cause immunosuppression and predispose poultry to other infectious diseases. The purpose of this study was diagnosis of MAREK’S DISEASE VIRUS as one of the causative agents for visceral tumors in CHICKENs using PCR. In order to perform this study, tumoral tissue sampling was collected from slaughter houses of Chaharmahal & Bakhtiari province. After DNA extraction from samples, Polymerase Chain Reaction (PCR) was performed using specific primers detecting 132bp tandem repeat and antigen Agene of MDV. Finally electrophoresis of PCR products was done on 1.5% agarose gel. The results showed that 62.86% of the total 280 nodul skin samples, tested for the presence of A genes of mark´s disease, were positive. In 40% of samples, 132 bp tandem repeat was also indentified. Since all the samples were tested have clinical symptoms of Marek’s disease, but the diagnosis of 62.86 percent infection based on PCR, it seems to be that other factors, except the MDV, have a role in tumor lesions in poultry.

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    Cite

    APA: Copy

    RABIEI, S., MOSHKELENI, S., DOOSTI, A., JAVAHERI KOUPAEI, M., & MAJLESI, A.R.. (2011). DIAGNOSIS OF MAREK’S DISEASE VIRUS (MDV) BY PCR. JOURNAL OF VETERINARY MICROBIOLOGY, 7(1 (22)), 35-39. SID. https://sid.ir/paper/152119/en

    Vancouver: Copy

    RABIEI S., MOSHKELENI S., DOOSTI A., JAVAHERI KOUPAEI M., MAJLESI A.R.. DIAGNOSIS OF MAREK’S DISEASE VIRUS (MDV) BY PCR. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2011;7(1 (22)):35-39. Available from: https://sid.ir/paper/152119/en

    IEEE: Copy

    S. RABIEI, S. MOSHKELENI, A. DOOSTI, M. JAVAHERI KOUPAEI, and A.R. MAJLESI, “DIAGNOSIS OF MAREK’S DISEASE VIRUS (MDV) BY PCR,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 7, no. 1 (22), pp. 35–39, 2011, [Online]. Available: https://sid.ir/paper/152119/en

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