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Information Journal Paper

Title

APPLICATION OF SILVER NANOPARTICLES TO CONTROL IN VITRO MICROBIAL CONTAMINATION AND RECOVERY OF THE SUGAR BEET DOUBLED HAPLOID PLANTLETS

Pages

  445-452

Abstract

 The purpose of this study was to examine the effectiveness of Nano silver colloid as a new antimicrobial agent against Erwinia in TISSUE CULTURE of SUGAR BEET for the recovery of the contaminated doubled haploid plantlets. The Nano silver colloidal solution of 4000 ppm was diluted in different concentrations (0, 50, 75, 100 and 125 ppm) and was added to the solid MS medium. In a factorial experiment, sample pre-washing (before subculture) with Nano silver solution to one–half of the plantlets was used. There were 10 treatment combinations replicated 5 times each. Number of the recovered plantlets and new shoots after 30 days were analyzed by means of analysis of variance technique based on a completely randomized design. Both factors were found to be significant. Results showed that Nano silver's behavior was different in liquid versus solid medium and affected by medium formulation. It was also indicated that the Nano silver did not remove completely bacteria from plantlets, even in as high as 120 ppm concentration, but drastically prevented bacterial growth and multiplication. A linear increase parallel to the Nano silver concentration occurred in the rate of recovery of the contaminated doubled haploid plantlets. Plantlets decay process was stopped in 125 ppm Nano silver concentration and effective recovery of them in in vitro condition occurred in the rate of 100%. Based on the results, it seems that the effectiveness of Nano silver on plantlet recovery is not just due to its antibacterial property, so its effect on the medium properties and biochemical processes need to be studied.

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    APA: Copy

    ROUZBEH, FARANAK, DAVOUDI, DARYOUSH, & MAJIDI, ESLAM. (2011). APPLICATION OF SILVER NANOPARTICLES TO CONTROL IN VITRO MICROBIAL CONTAMINATION AND RECOVERY OF THE SUGAR BEET DOUBLED HAPLOID PLANTLETS. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 42(3), 445-452. SID. https://sid.ir/paper/153684/en

    Vancouver: Copy

    ROUZBEH FARANAK, DAVOUDI DARYOUSH, MAJIDI ESLAM. APPLICATION OF SILVER NANOPARTICLES TO CONTROL IN VITRO MICROBIAL CONTAMINATION AND RECOVERY OF THE SUGAR BEET DOUBLED HAPLOID PLANTLETS. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2011;42(3):445-452. Available from: https://sid.ir/paper/153684/en

    IEEE: Copy

    FARANAK ROUZBEH, DARYOUSH DAVOUDI, and ESLAM MAJIDI, “APPLICATION OF SILVER NANOPARTICLES TO CONTROL IN VITRO MICROBIAL CONTAMINATION AND RECOVERY OF THE SUGAR BEET DOUBLED HAPLOID PLANTLETS,” IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 42, no. 3, pp. 445–452, 2011, [Online]. Available: https://sid.ir/paper/153684/en

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