OPTIMIZATION OF SILYBUM MARIANUM TISSUE CULTURE FOR PRODUCTION OF MEDICINAL FLAVONOLIGNAN

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AREKHI S.; AGHDASI M.; KHALAFI M.

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Journal: Journal of Plant Production Research Year:2012 | Volume:19 | Issue:2 Page(s): 69-87

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Title

OPTIMIZATION OF SILYBUM MARIANUM TISSUE CULTURE FOR PRODUCTION OF MEDICINAL FLAVONOLIGNAN

Author(s)

AREKHI S. | AGHDASI M. | KHALAFI M. | Issue Writer Certificate

Keywords

SILYBUM MARIANUMQ1

SILYMARINQ1

TISSUE CULTUREQ2

HORMONEQ2

EXPLANTSQ2

FLAVONOLIGNANQ2

Abstract

The SILYBUM MARIANUM is the dicotyledonous herbs of the Asteraceae Family that is important in medicinal industry. The biological active compound of SILYBUM MARIANUMis a mixture of several FLAVONOLIGNANs generally known as the SILYMARIN. These FLAVONOLIGNANs are used for treatment of various liver diseases, hepatitis, diabetes, heart diseases, cancer, blood cholesterol and etc. The purpose of this work was optimizing of Silybium marianum TISSUE CULTURE for SILYMARIN production. In the current work, the effect of 2, 4-D, Kin and NAA HORMONEs were investigated on callus formation in root, leaf and shoot EXPLANTS. Finally the best HORMONE concentration was determined for SILYMARIN production. The experiment performed in complete randomized test with three replicates. The results showed that the highest percentage of callus formation (%98) was observed at 1 & 1.5 mg/l of 2, 4- D and Kin HORMONEs in root EXPLANTS. Also maximum percentage of callus formation (%97) was observed at 1.5 & 1.5 mg/l of NAA and Kin HORMONE treatment in root EXPLANTS. In the most treatments, the color of callus was light yellow to light green and their physical structures were soft. FLAVONOLIGNAN measurement showed that the maximum percentage of SILYMARIN (14.4%) was observed at 0.5 & 1 mg/l of 2, 4-D and Kin HORMONEs in calluses from root EXPLANTS. Meanwhile, the highest percentage of SILYMARIN (9.68%) was achieved at 1.5 & 1 mg/l of NAA and Kin in calluses from root EXPLANTS.

Cites

INDUCTION OF ROOT FORMATION TO PRODUCE SILYMARIN IN SILYBIUM MARIANUM PLANT IN TISSUE CULTURE CONDITION

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APA: Copy

AREKHI, S., AGHDASI, M., & KHALAFI, M.. (2012). OPTIMIZATION OF SILYBUM MARIANUM TISSUE CULTURE FOR PRODUCTION OF MEDICINAL FLAVONOLIGNAN. JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), 19(2), 69-87. SID. https://sid.ir/paper/155779/en

Vancouver: Copy

AREKHI S., AGHDASI M., KHALAFI M.. OPTIMIZATION OF SILYBUM MARIANUM TISSUE CULTURE FOR PRODUCTION OF MEDICINAL FLAVONOLIGNAN. JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES)[Internet]. 2012;19(2):69-87. Available from: https://sid.ir/paper/155779/en

IEEE: Copy

S. AREKHI, M. AGHDASI, and M. KHALAFI, “OPTIMIZATION OF SILYBUM MARIANUM TISSUE CULTURE FOR PRODUCTION OF MEDICINAL FLAVONOLIGNAN,” JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), vol. 19, no. 2, pp. 69–87, 2012, [Online]. Available: https://sid.ir/paper/155779/en

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