THE INFLUENCE OF LIGHT, TEMPERATURE AND MEDIUM PH ON FLAVONOLIGNAN FORMATION IN SILYBUM MARIANUM CELL CULTURES

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ASGHARI G.; GHASAMI DEHKORDI N.; SHAFIEI M.

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Journal: JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS) Year:2003 | Volume:7 | Issue:Supplement 2 Page(s): 100-102

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Title

THE INFLUENCE OF LIGHT, TEMPERATURE AND MEDIUM PH ON FLAVONOLIGNAN FORMATION IN SILYBUM MARIANUM CELL CULTURES

Author(s)

ASGHARI G. | GHASAMI DEHKORDI N. | SHAFIEI M. | Issue Writer Certificate

Keywords

FLAVONOLIGNANQ4

SILYBUM MARIANUMQ4

CELL CUTLUREQ3

LIGHTQ4

TEMPERATUREQ2

Abstract

Introduction. SILYBUM MARIANUM has been recognized as an antihepatotoxic plant. The active constituents of S. marianum include a group of FLAVONOLIGNANs known collectively as silymarin. Silymarin production by cell culture of S. marianum has been considered as a high LIGHTed research. The succesful exploitation of S. marianum cultures requires an understanding of the physical and biochemical factors affecting FLAVONOLIGNAN production.Methods. Callus cultures of S. marianum were established by transferring seedling, grown on solidified MS medium supplemented with 1 ppm 2,4-Dichloro phenoxy acetic acid and 0.1 ppm kinetin. Callus cultures were subjected to various TEMPERATUREs, LIGHT treatments, and culture medium pHs. Cultures were harvested after 28 days, then deried, and extracted with methanol. Qualitative and quantitative analysis of FLAVONOLIGNANs using TLC and spectrophotometric methods were carried out on the cells.Results. Higher levels of FLAVONOLIGNAN accumulation under dark rather than LIGHT and LIGHT-dark conditions were produced. The same results were observed at 20°C compared to 25°C or 30°C. Also, the culture medium pH 7 was superior to pH 5.6 or pH 9 for FLAVONOLIGNAN production.Discussion. The results demonstrate the influnce of TEMPERATURE, medium pH, and LIGHT treatment on FLAVONOLIGNAN formation in S. marianum callus cultures.

Cites

THE INFLUENCE OF FRUCTOSE, GLUCOSE AND SUCROSE ON FLAVONOLIGNANS FORMATION IN SILYBUM MARIANUM CALLUS CULTURE

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APA: Copy

ASGHARI, G., GHASAMI DEHKORDI, N., & SHAFIEI, M.. (2003). THE INFLUENCE OF LIGHT, TEMPERATURE AND MEDIUM PH ON FLAVONOLIGNAN FORMATION IN SILYBUM MARIANUM CELL CULTURES. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), 7(Supplement 2), 100-102. SID. https://sid.ir/paper/26685/en

Vancouver: Copy

ASGHARI G., GHASAMI DEHKORDI N., SHAFIEI M.. THE INFLUENCE OF LIGHT, TEMPERATURE AND MEDIUM PH ON FLAVONOLIGNAN FORMATION IN SILYBUM MARIANUM CELL CULTURES. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS)[Internet]. 2003;7(Supplement 2):100-102. Available from: https://sid.ir/paper/26685/en

IEEE: Copy

G. ASGHARI, N. GHASAMI DEHKORDI, and M. SHAFIEI, “THE INFLUENCE OF LIGHT, TEMPERATURE AND MEDIUM PH ON FLAVONOLIGNAN FORMATION IN SILYBUM MARIANUM CELL CULTURES,” JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), vol. 7, no. Supplement 2, pp. 100–102, 2003, [Online]. Available: https://sid.ir/paper/26685/en

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