Evaluation of the Effects of Adipose Derived Mesenchymal Stem Cells Cultured on Decellularized Amniotic Membrane in Wound Healing of Distal Part of the Limbs in Horse

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Khashjoori Bassim Kadhim; Ghamsari Seyed mehdi; DEHGHAN MOHAMMAD MEHDI; Farzad Mohajeri Saeed; GHOLAMI HOSSEIN; GOLSHAHI HANNANEH

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Journal: Iranian Journal of Veterinary Medicine Year:2019 | Volume:13 | Issue:1 Page(s): 11-25

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Title

Evaluation of the Effects of Adipose Derived Mesenchymal Stem Cells Cultured on Decellularized Amniotic Membrane in Wound Healing of Distal Part of the Limbs in Horse

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Keywords

Amniotic membraneQ1

Distal limbQ1

HorseQ1

Mesenchymal stem cellsQ1

Wounds healingQ1

Abstract

BACKGROUND: Equine wounds, particularly those involving the distal portion of the limbs, often undergo prolonged complex healing and may enter a non-healing state with obvious financial and welfare implications. Poor blood supply and lower levels of oxygen to the lower limbs lead to imbalance of growth factors related to wound healing. Decellular Amniotic membrane has been widely used in soft tissue engineering especially skin regeneration due to its biological and mechanical properties. It is used as a scaffold for treatment of partial full thickness wound dressing. OBJECTIVES: Evaluation of the effects adipose derived mesenchymal cells (ADMSCs) cultured on decellularized Amniotic membrane (DAM) in wound healing of distal part of equine limb. METHODS: Stem cells were isolated from adipose tissue in the base of the tail and then cultured and seeded on bovine decellular Amniotic membrane. Full thickness excision wounds (2x3 cm) were created on the mid-lateral of all metatarsus of each Horse aseptically. The wounds were classified into four groups: the first one covered by DAM seeded by ADMSCs, the second one dressed with only DAM, the third one treated with1% silver sulfadiazine, and the last one as control with no treatment. Digital photographs of wounds were obtained at each bandage change while biopsy samples were obtained for histopathology at days 5, 15 and 30 after treatment. RESULTS: Wounds treated with DAM/ADMSCs significantly demonstrated promotion of wound healing in geometric analysis of total wound area, epithelialization and wound contraction compared with the other groups (P<0. 05). Microscopically, re-epithelization of wounds covered by DAM/ADMSCs and DAM at day 5 was significantly more than Control group (P<0. 05). Fibroplasia in wounds treated with DAM/ADMSCs and DAM was higher than the group that received sulfadiazine (P<0. 05). CONCLUSIONS: These results indicate positive effects of using DAM to improve the quality and speed of wound healing. Use of DAM as a scaffold for ADMSCs shows better results.

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APA: Copy

Khashjoori, Bassim Kadhim, Ghamsari, Seyed mehdi, DEHGHAN, MOHAMMAD MEHDI, Farzad Mohajeri, Saeed, GHOLAMI, HOSSEIN, & GOLSHAHI, HANNANEH. (2019). Evaluation of the Effects of Adipose Derived Mesenchymal Stem Cells Cultured on Decellularized Amniotic Membrane in Wound Healing of Distal Part of the Limbs in Horse. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 13(1 ), 11-25. SID. https://sid.ir/paper/158044/en

Vancouver: Copy

Khashjoori Bassim Kadhim, Ghamsari Seyed mehdi, DEHGHAN MOHAMMAD MEHDI, Farzad Mohajeri Saeed, GHOLAMI HOSSEIN, GOLSHAHI HANNANEH. Evaluation of the Effects of Adipose Derived Mesenchymal Stem Cells Cultured on Decellularized Amniotic Membrane in Wound Healing of Distal Part of the Limbs in Horse. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2019;13(1 ):11-25. Available from: https://sid.ir/paper/158044/en

IEEE: Copy

Bassim Kadhim Khashjoori, Seyed mehdi Ghamsari, MOHAMMAD MEHDI DEHGHAN, Saeed Farzad Mohajeri, HOSSEIN GHOLAMI, and HANNANEH GOLSHAHI, “Evaluation of the Effects of Adipose Derived Mesenchymal Stem Cells Cultured on Decellularized Amniotic Membrane in Wound Healing of Distal Part of the Limbs in Horse,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 13, no. 1 , pp. 11–25, 2019, [Online]. Available: https://sid.ir/paper/158044/en

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