Optimization of direct somatic embryogenesis induction and plantlet regeneration in European Pear

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Ameri Atefe; Davarynejad Golamhossein; MOSHTAGHI NASRIN; TEHRANIFAR ALI

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Journal: Journal of Plant Biological Sciences Year:2019 | Volume:11 | Issue:39 Page(s): 43-58

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Title

Optimization of direct somatic embryogenesis induction and plantlet regeneration in European Pear

Author(s)

Ameri Atefe | Davarynejad Golamhossein | MOSHTAGHI NASRIN | TEHRANIFAR ALI | Issue Writer Certificate

Keywords

2Q1

4-DQ2

Fe-EDDHAQ1

Ghosi cultivarQ1

MycorrhizaeQ1

Abstract

To evaluate somatic embryogenesis (SE) induction, mature, immature cotyledon and endosperm explants of Natanzi, Ghosi, Dar Gazi, and Williams cultivars obtained from 40-100 days after pollination, were cultured into the White, SH, and MS media containing 2. 3, 9 and 18 μ M of 2, 4-D. Then, to enhance the efficiency of direct SE, Ghosi cultivar endosperm as the preferable explant from previous experiment, was cultured in MS medium containing 0 to 2 μ M of 2, 4-D and 0 to 9 μ M of TDZ. To germinate and plant recovery, MS medium with different treatments consisted of control, 150 mg/L Fe-EDDHA, 2 mg/L AgNO3, and 1mg/L GA3 were used. Finally, for plant adaptation, two successive experiments consisted of using Mycorrhizae (Glomus mosseae) treatment at 0 and 0. 25 g/Kg levels, and then applying two fertigation procedures, were separately carried out. The results revealed, the only endosperm of Ghosi cultivar in MS medium produced direct somatic embryos. 0. 5 μ M of 2, 4-D had the highest frequency of somatic embryos production (12. 66%). The highest frequency of normal embryo obtained in 0. 5 μ M 2, 4-D (71%) and 0. 5 μ M 2, 4-D + 4. 5 μ M TDZ (70%). The highest germination (9. 66%) and plant recovery (12%) obtained in Fe-EDDHA. In the adaptation phase, the usage of Mycorrhizae and the second procedure of fertigation were known preferable. Overall, the method presented in this study can be used in molecular studies of this cultivar.

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APA: Copy

Ameri, Atefe, Davarynejad, Golamhossein, MOSHTAGHI, NASRIN, & TEHRANIFAR, ALI. (2019). Optimization of direct somatic embryogenesis induction and plantlet regeneration in European Pear. IRANIAN JOURNAL OF PLANT BIOLOGY, 11(39 ), 43-58. SID. https://sid.ir/paper/160097/en

Vancouver: Copy

Ameri Atefe, Davarynejad Golamhossein, MOSHTAGHI NASRIN, TEHRANIFAR ALI. Optimization of direct somatic embryogenesis induction and plantlet regeneration in European Pear. IRANIAN JOURNAL OF PLANT BIOLOGY[Internet]. 2019;11(39 ):43-58. Available from: https://sid.ir/paper/160097/en

IEEE: Copy

Atefe Ameri, Golamhossein Davarynejad, NASRIN MOSHTAGHI, and ALI TEHRANIFAR, “Optimization of direct somatic embryogenesis induction and plantlet regeneration in European Pear,” IRANIAN JOURNAL OF PLANT BIOLOGY, vol. 11, no. 39 , pp. 43–58, 2019, [Online]. Available: https://sid.ir/paper/160097/en

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