COMPARATIVE STUDY OF TROPANE ALKALOIDS PRODUCTION IN HAIRY ROOTS AND PLANTLET CULTURES OF ATROPA BELLADONNA L. BY SALICYLIC ACID TREATMENTS

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AHMADIAN CHASHMI N.; SHARIFI MOZAFAR; KARIMI FARAH; RAHNAMA H.

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Journal: Journal of Plant Biological Sciences Year:2010 | Volume:2 | Issue:1 (SEQUENCE 3) Page(s): 63-76

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Title

COMPARATIVE STUDY OF TROPANE ALKALOIDS PRODUCTION IN HAIRY ROOTS AND PLANTLET CULTURES OF ATROPA BELLADONNA L. BY SALICYLIC ACID TREATMENTS

Author(s)

AHMADIAN CHASHMI N. | SHARIFI MOZAFAR | KARIMI FARAH | RAHNAMA H. | Issue Writer Certificate

Keywords

TROPANE ALKALOIDSQ3

HAIRY ROOTSQ2

SALICYLIC ACIDQ2

ATROPA BELLADONNAQ3

Abstract

Most Solanaceae plants produce a range of biologically important alkaloids including nicotine and TROPANE ALKALOIDS, such as hyoscyamine (atropine) and scopolamine. These alkaloids are used for their medicinal properties. ATROPA BELLADONNA is a medicinally important herbaceous plant that produces high amount of TROPANE ALKALOIDS in roots. In this research, ATROPA BELLADONNA explants were obtained from sterilized seedlings in the modified Murashige and Skoog (MS) solid medium. The seeds were collected from Vaz and Garmestan regions of Mazandaran Province, Iran. Explants were cultured for four weeks on a modified MS solid medium, containing different concentrations of SALICYLIC ACID (0, 0.01, 0.1 and 1 mM). In addition, HAIRY ROOTS of ATROPA BELLADONNA that was established by transformation with Agrobacterium rhizogenes, were cultured in MS medium for two weeks. Then the production of two TROPANE ALKALOIDS, atropine and scopolamine, in different parts of neoformed plants were assayed by HPLC method. Generally, tropan alkaloids content in HAIRY ROOTS was considerably higher compared to those contents in plants. Although Garmestan population was better than Vaz group, the amount of atropine in HAIRY ROOTS was influenced by SALICYLIC ACID as high as 5 to 35 times of produced atropine in different parts of plants. The scopolamine content in the HAIRY ROOTS was 2-30 times higher than in plant organs. In conclusion, it is suggested that, hairy root lines can be used as a replacement of plants in further studies and for TROPANE ALKALOIDS production in economical and commercial scales.

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بررسی تولید تروپان آلکالوئیدها از طریق کشت ریشه های تراریخته گیاه شابیزک(Atrops belladonna) با استفاده از Agrobacterium rhizogenes (همایش)

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APA: Copy

AHMADIAN CHASHMI, N., SHARIFI, MOZAFAR, KARIMI, FARAH, & RAHNAMA, H.. (2010). COMPARATIVE STUDY OF TROPANE ALKALOIDS PRODUCTION IN HAIRY ROOTS AND PLANTLET CULTURES OF ATROPA BELLADONNA L. BY SALICYLIC ACID TREATMENTS. IRANIAN JOURNAL OF PLANT BIOLOGY, 2(1 (SEQUENCE 3)), 63-76. SID. https://sid.ir/paper/160105/en

Vancouver: Copy

AHMADIAN CHASHMI N., SHARIFI MOZAFAR, KARIMI FARAH, RAHNAMA H.. COMPARATIVE STUDY OF TROPANE ALKALOIDS PRODUCTION IN HAIRY ROOTS AND PLANTLET CULTURES OF ATROPA BELLADONNA L. BY SALICYLIC ACID TREATMENTS. IRANIAN JOURNAL OF PLANT BIOLOGY[Internet]. 2010;2(1 (SEQUENCE 3)):63-76. Available from: https://sid.ir/paper/160105/en

IEEE: Copy

N. AHMADIAN CHASHMI, MOZAFAR SHARIFI, FARAH KARIMI, and H. RAHNAMA, “COMPARATIVE STUDY OF TROPANE ALKALOIDS PRODUCTION IN HAIRY ROOTS AND PLANTLET CULTURES OF ATROPA BELLADONNA L. BY SALICYLIC ACID TREATMENTS,” IRANIAN JOURNAL OF PLANT BIOLOGY, vol. 2, no. 1 (SEQUENCE 3), pp. 63–76, 2010, [Online]. Available: https://sid.ir/paper/160105/en

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