THE EFFECT OF CHOLORATE MICROMOLAR CONCENTRATIONS ON HATS NITRATE UPTAKE IN ARABIDOPSIS THALINA

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ZOUFAN PARZHAK; SHARIATI MANSOUR

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Paper Information

Journal: Journal of Plant Biological Sciences Year:2010 | Volume:2 | Issue:3 (SEQUENCE 5) Page(s): 25-36

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Title

THE EFFECT OF CHOLORATE MICROMOLAR CONCENTRATIONS ON HATS NITRATE UPTAKE IN ARABIDOPSIS THALINA

Author(s)

ZOUFAN PARZHAK | SHARIATI MANSOUR | Issue Writer Certificate

Keywords

ARABIDOPSISQ2

HIGH AFFINITY TRANSPORT SYSTEMQ3

CHOLORATEQ3

NITRATEQ2

Abstract

The effect of CHOLORATE different concentrations between 0 to 100 µM on HIGH AFFINITY TRANSPORT SYSTEM activity using ARABIDOPSIS thaliana mutants atnrt2.1, atnrt2.4 and chl1-5 and transformants in NRT2.1 gene in two research sections was studied. In the first section, two pH 6 and 6.5 were used in a medium of free NITRATE. 3 different NITRATE and CHOLORATE concentrations including 0, 25 and 50 µM with pH 6.5 were selected during second section. All seeds were cultured on solid medium and maintained in a controlled-environment condition. After 7 days, in order to gain further insight into the role of NITRATE HIGH AFFINITY TRANSPORT SYSTEM, percentage of full expanded leaflets as indicator of HIGH AFFINITY TRANSPORT SYSTEM activity was investigated. The results showed the superiority of atnrt2.1 mutant in comparison with the other genotypes, especially on higher concentrations of CHOLORATE, either with NITRATE or without NITRATE. Deregulation of AtNRT2.4 and AtCHL1 genes was not conducive to significant resistance. On the other hand, upregulation of NpNRT2.1 gene in atnrt2.1 mutant decreased percentage of full expanded leaflets. Based on these results, it was confirmed that AtNRT2.1 gene had key and important role in NITRATE uptake by HIGH AFFINITY TRANSPORT SYSTEM.

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References

PHYSIOLOGICAL AND MOLECULAR RESPONSES OF NRT2.4 TRANSPORTER UNDER N-STARVATION CONDITIONS IN ARABIDOPSIS THALIANA

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APA: Copy

ZOUFAN, PARZHAK, & SHARIATI, MANSOUR. (2010). THE EFFECT OF CHOLORATE MICROMOLAR CONCENTRATIONS ON HATS NITRATE UPTAKE IN ARABIDOPSIS THALINA. IRANIAN JOURNAL OF PLANT BIOLOGY, 2(3 (SEQUENCE 5)), 25-36. SID. https://sid.ir/paper/160113/en

Vancouver: Copy

ZOUFAN PARZHAK, SHARIATI MANSOUR. THE EFFECT OF CHOLORATE MICROMOLAR CONCENTRATIONS ON HATS NITRATE UPTAKE IN ARABIDOPSIS THALINA. IRANIAN JOURNAL OF PLANT BIOLOGY[Internet]. 2010;2(3 (SEQUENCE 5)):25-36. Available from: https://sid.ir/paper/160113/en

IEEE: Copy

PARZHAK ZOUFAN, and MANSOUR SHARIATI, “THE EFFECT OF CHOLORATE MICROMOLAR CONCENTRATIONS ON HATS NITRATE UPTAKE IN ARABIDOPSIS THALINA,” IRANIAN JOURNAL OF PLANT BIOLOGY, vol. 2, no. 3 (SEQUENCE 5), pp. 25–36, 2010, [Online]. Available: https://sid.ir/paper/160113/en

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