USE OF MICROSATELLITE ASSOCIATED WITH RESISTANCE TO IHN VIRUS IN SCREENING OF RAINBOW TROUT BROOD STOCK POPULATION (ONCORHYNCHUS MYKISS)

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MOHAMMADTAHERI M; MIRVAGHEFI A.; FARAHMAND H.; MOJAZI AMIRI B.; GHOVATI N.; KHALILI B.

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Journal: Journal of Fisheries Year:2010 | Volume:63 | Issue:2 Page(s): 111-120

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Title

USE OF MICROSATELLITE ASSOCIATED WITH RESISTANCE TO IHN VIRUS IN SCREENING OF RAINBOW TROUT BROOD STOCK POPULATION (ONCORHYNCHUS MYKISS)

Author(s)

Keywords

RAINBOW TROUTQ2

MICROSATELLITEQ2

RESISTANCE TO IHN VIRUSQ3

GENETIC SCREENINGQ3

MARKER ASSISTED SELECTION (MAS)Q3

Abstract

The aim of present study was to screen RAINBOW TROUT broodstock population of Kelardasht Reproduction and Rearing Center, with emphasis on presence and non-presence of MICROSATELLITE markers associated with RESISTANCE TO IHN VIRUS. For this purpose, 92 samples of caudal fin tissue from Kelardasht RAINBOW TROUT brood stock population were obtained and genomic DNA of these samples were extracted. Two MICROSATELLITE loci include Ssa7NVH, Ssa94NVH were amplified using the polymerase chain reaction (PCR) with their specific primers. This study showed that presence of these MICROSATELLITE markers in this brood stock population were 98.49%, 95.46% and the number of polymorphs for those were 4, 11 respectively. According to sex-differentiation, the presence of these MICROSATELLITE markers based on Ssa94NVH in male and female were 92.4 & 97.5 and based on Ssa7VNH were 100 & 97.5 respectively.

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APA: Copy

MOHAMMADTAHERI, M,, MIRVAGHEFI, A., FARAHMAND, H., MOJAZI AMIRI, B., GHOVATI, N., & KHALILI, B.. (2010). USE OF MICROSATELLITE ASSOCIATED WITH RESISTANCE TO IHN VIRUS IN SCREENING OF RAINBOW TROUT BROOD STOCK POPULATION (ONCORHYNCHUS MYKISS). JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), 63(2), 111-120. SID. https://sid.ir/paper/162132/en

Vancouver: Copy

MOHAMMADTAHERI M,, MIRVAGHEFI A., FARAHMAND H., MOJAZI AMIRI B., GHOVATI N., KHALILI B.. USE OF MICROSATELLITE ASSOCIATED WITH RESISTANCE TO IHN VIRUS IN SCREENING OF RAINBOW TROUT BROOD STOCK POPULATION (ONCORHYNCHUS MYKISS). JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES)[Internet]. 2010;63(2):111-120. Available from: https://sid.ir/paper/162132/en

IEEE: Copy

M, MOHAMMADTAHERI, A. MIRVAGHEFI, H. FARAHMAND, B. MOJAZI AMIRI, N. GHOVATI, and B. KHALILI, “USE OF MICROSATELLITE ASSOCIATED WITH RESISTANCE TO IHN VIRUS IN SCREENING OF RAINBOW TROUT BROOD STOCK POPULATION (ONCORHYNCHUS MYKISS),” JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), vol. 63, no. 2, pp. 111–120, 2010, [Online]. Available: https://sid.ir/paper/162132/en

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