RECOMBINANT EXPRESSION OF HUMAN EPIDERMAL GROWTH FACTOR IN E. COLI AND ASSESSMENT OF ITS EFFECT IN NIH-3T3 CELL LINE

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BAKHSHI M.; EBRAHIMI F.; HONARI H.; HAJIZADEH A.; AREFPOOR TORABI M.A.

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Journal: Advanced Defense Science and Technology Year:2012 | Volume:2 | Issue:4 (6) Page(s): 325-332

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Title

RECOMBINANT EXPRESSION OF HUMAN EPIDERMAL GROWTH FACTOR IN E. COLI AND ASSESSMENT OF ITS EFFECT IN NIH-3T3 CELL LINE

Author(s)

Keywords

RECOMBINANT HEGFQ3

SYNTHETIC GENEQ3

E. COLIQ3

NIH-3T3 CELL LINEQ3

Abstract

The EGF family include13 members and the hEGF as founding member of this family involves in epidermal wound healing. Epidermal growth factor is an essential polypeptide for wound healing and promoting mitotic division. In this study, human epidermal growth factor gene was adapted from gene bank and its codons were optimized according to codon preference of E. COLI BL21. The gene was prepared as synthetic construct in pET21a (+). Then the recombinant construct was transferred to E. COLI and hEGF gene in the vector was expressed under the control of inducible T7 promoter. Purification was done using affinity chromatography. The bioassay results of three different doses showed that recombinant protein has significant effects on growth of NIH-3T3 CELL LINE.

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APA: Copy

BAKHSHI, M., EBRAHIMI, F., HONARI, H., HAJIZADEH, A., & AREFPOOR TORABI, M.A.. (2012). RECOMBINANT EXPRESSION OF HUMAN EPIDERMAL GROWTH FACTOR IN E. COLI AND ASSESSMENT OF ITS EFFECT IN NIH-3T3 CELL LINE. (JOURNAL OF ADVANCED DEFENCE SCIENCE AND TECHNOLOGY) JOURNAL OF PASSIVE DEFENCE SCIENCE AND TECHNOLOGY, 2(4 (6)), 325-332. SID. https://sid.ir/paper/167304/en

Vancouver: Copy

BAKHSHI M., EBRAHIMI F., HONARI H., HAJIZADEH A., AREFPOOR TORABI M.A.. RECOMBINANT EXPRESSION OF HUMAN EPIDERMAL GROWTH FACTOR IN E. COLI AND ASSESSMENT OF ITS EFFECT IN NIH-3T3 CELL LINE. (JOURNAL OF ADVANCED DEFENCE SCIENCE AND TECHNOLOGY) JOURNAL OF PASSIVE DEFENCE SCIENCE AND TECHNOLOGY[Internet]. 2012;2(4 (6)):325-332. Available from: https://sid.ir/paper/167304/en

IEEE: Copy

M. BAKHSHI, F. EBRAHIMI, H. HONARI, A. HAJIZADEH, and M.A. AREFPOOR TORABI, “RECOMBINANT EXPRESSION OF HUMAN EPIDERMAL GROWTH FACTOR IN E. COLI AND ASSESSMENT OF ITS EFFECT IN NIH-3T3 CELL LINE,” (JOURNAL OF ADVANCED DEFENCE SCIENCE AND TECHNOLOGY) JOURNAL OF PASSIVE DEFENCE SCIENCE AND TECHNOLOGY, vol. 2, no. 4 (6), pp. 325–332, 2012, [Online]. Available: https://sid.ir/paper/167304/en

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