THE STUDY OF SUGARCANE ANIUPLOIDY VARIATIONS IN VITRO CULTURE

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SADAT SH.; SOLTANI HOVEIZEH M.; ETA A.

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Journal: CROP PHYSIOLOGY Year:2010 | Volume:2 | Issue:1 Page(s): 129-142

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Information Journal Paper

Title

THE STUDY OF SUGARCANE ANIUPLOIDY VARIATIONS IN VITRO CULTURE

Author(s)

SADAT SH. | SOLTANI HOVEIZEH M. | ETA A. | Issue Writer Certificate

Keywords

SUGARCANEQ2

CALLUSQ2

REGENERATIONQ2

ROOT FORMATIONQ2

ACCLIMATIZATIONQ2

Abstract

In order to study of SUGARCANE (Saccharum officinarum L.) aniuploidy in vitro culture, disinfection of initial leaves of SUGARCANE variety CP69-1062 was studied using six treatments in a completely randomized design (CRD) with five replications. Sequential applications of 20% sodium hypochlorite for 10 minutes and 0.1% mercuric chloride for 3 minutes and 30 seconds had the best results on disinfection of initial leaves. Six CALLUS induction treatments were evaluated through completely randomized design with four replications. Murashig and skoog (MS) based medium supplemented with 2.5 mgl-1 2, 4-D had the best results for CALLUS induction of initial leaves. REGENERATION of calli was studied using five treatments in a completely randomized design with five replications. MS medium supplemented with 1 mgl-1 BAP and 0.5 mgl-1 IBA and also 2 mgl-1 BAP and 0.5 mgl-1 NAA were more efficient on REGENERATION.Root formation was studied using five treatments in a completely randomized design with four replications. MS medium supplemented with 3.5 mgl-1 NAA was found as the best for root induction treatment. Cytogenetics studies were performed 45 days after transmission of regenerants to the soil (71% ACCLIMATIZATION).32.5% of selected plantlets demonstrated aniuploidy in their root meristemic cells.

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References

KARYOLOGICAL EVALUATION OF FIVE HYBRID CULTIVARS OF SUGARCANE (SACCHARUM OFFICINARUM L.)

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APA: Copy

SADAT, SH., SOLTANI HOVEIZEH, M., & ETA, A.. (2010). THE STUDY OF SUGARCANE ANIUPLOIDY VARIATIONS IN VITRO CULTURE. CROP PHYSIOLOGY, 2(1), 129-142. SID. https://sid.ir/paper/174437/en

Vancouver: Copy

SADAT SH., SOLTANI HOVEIZEH M., ETA A.. THE STUDY OF SUGARCANE ANIUPLOIDY VARIATIONS IN VITRO CULTURE. CROP PHYSIOLOGY[Internet]. 2010;2(1):129-142. Available from: https://sid.ir/paper/174437/en

IEEE: Copy

SH. SADAT, M. SOLTANI HOVEIZEH, and A. ETA, “THE STUDY OF SUGARCANE ANIUPLOIDY VARIATIONS IN VITRO CULTURE,” CROP PHYSIOLOGY, vol. 2, no. 1, pp. 129–142, 2010, [Online]. Available: https://sid.ir/paper/174437/en

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