REGENERATION OF TWO COMMERCIAL SUGARCANE CULTIVARS (CP48-103 AND CP69-1062) FROM TERMINAL LEAVES DERIVED EXPLANTS

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KALANTARHORMOZI S.; SIAHPOOSH M.R.; RAJABI MEMARI H.; HAMDI H.; SHOMEILI M.; HAMOUDI J.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2015 | Volume:7 | Issue:1 Page(s): 155-174

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Title

REGENERATION OF TWO COMMERCIAL SUGARCANE CULTIVARS (CP48-103 AND CP69-1062) FROM TERMINAL LEAVES DERIVED EXPLANTS

Author(s)

Keywords

REGENERATIONQ2

STERILIZATIONQ2

CALLUSQ2

TISSUE CULTUREQ3

KINETINQ2

SUGARCANEQ2

Abstract

Sugarcane is one of the most important industrial plants. There are many ways to improve the agronomic traits of this plant, such as genetic engineering and gene transfer methods. These modern techniques depend on optimizing the REGENERATION of plants from CALLUS tissue. In this study, we attempted to optimize all different stages of TISSUE CULTURE for regenerating the plants from CALLUS tissue derived from terminal roll lives of two commercial SUGARCANE cultivars, CP48-10 and CP69-1062. Two types of cutting were applied, ring cutting and half-cylinder cutting from roll leaves. Among these cutting types, ring cutting from the roll leaves gives better results. Using solid MS medium with 3 mg per liter 2, 4-D could attain the maximum CALLUS production. To produce shoots from CALLUS, the hormone combination of 1 mg per liter BAP and 2 mg per liter KINETIN showed the best response. Seedlings produced in MS medium containing 5 mg per liter IBA, 1 mg per liter KINETIN and 3 mg per liter NAA accompanied by 3 g per liter activated charcoal rendered the most root production. In the treatments with activated charcoal, the regenerated seedlings had superiority for the traits, days to root emergence, root length and number of roots in plants.

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APA: Copy

KALANTARHORMOZI, S., SIAHPOOSH, M.R., RAJABI MEMARI, H., HAMDI, H., SHOMEILI, M., & HAMOUDI, J.. (2015). REGENERATION OF TWO COMMERCIAL SUGARCANE CULTIVARS (CP48-103 AND CP69-1062) FROM TERMINAL LEAVES DERIVED EXPLANTS. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 7(1), 155-174. SID. https://sid.ir/paper/224589/en

Vancouver: Copy

KALANTARHORMOZI S., SIAHPOOSH M.R., RAJABI MEMARI H., HAMDI H., SHOMEILI M., HAMOUDI J.. REGENERATION OF TWO COMMERCIAL SUGARCANE CULTIVARS (CP48-103 AND CP69-1062) FROM TERMINAL LEAVES DERIVED EXPLANTS. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2015;7(1):155-174. Available from: https://sid.ir/paper/224589/en

IEEE: Copy

S. KALANTARHORMOZI, M.R. SIAHPOOSH, H. RAJABI MEMARI, H. HAMDI, M. SHOMEILI, and J. HAMOUDI, “REGENERATION OF TWO COMMERCIAL SUGARCANE CULTIVARS (CP48-103 AND CP69-1062) FROM TERMINAL LEAVES DERIVED EXPLANTS,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 7, no. 1, pp. 155–174, 2015, [Online]. Available: https://sid.ir/paper/224589/en

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