THE COMPARISON OF DIFFERENT METHOD CULTURES OF HARMFUL DINOFLAGEL COCHLODINIUM POLYKRIKOIDES AT LABORATORY CONDITION

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GHORBANI VAGHEIE REZA; ASADI ALIREZA; PAIGOZAR AKBAR

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Journal: JOURNAL OF MARINE BIOLOGY Year:2013 | Volume:5 | Issue:19 Page(s): 79-88

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Title

THE COMPARISON OF DIFFERENT METHOD CULTURES OF HARMFUL DINOFLAGEL COCHLODINIUM POLYKRIKOIDES AT LABORATORY CONDITION

Author(s)

GHORBANI VAGHEIE REZA | ASADI ALIREZA | PAIGOZAR AKBAR | Issue Writer Certificate

Keywords

COCHLODINIUM POLYKRIKOIDESQ2

CULTURE MEDIUMQ2

CELL DENSITYQ3

LABORATORY CONDITIONQ3

PERSIAN GULFQ2

Abstract

Cochlodinium is a dinoflagel that it’s bloom in the coastal water of worlds and caused to fish mortality. Cochlodinium was detected in the PERSIAN GULF since 2008.After purification of C. polykricoides using light, it cultured using medium of f/2 (modified), Guilard (f/2-Si), TMRL, Conway and ESM. Cochlodinium cultured in tubes, erlanmeyer with volume of 100-1000 ml and aquariums with the volume of 16 liters (each with 10 liter water) with three replicates in each treatment, in the water salinity of 32 ppt, water tempreture of 25 0C, light intensity of 5000 lux and 12 hours dark and 12 hours light. The maximum CELL DENSITY reached to 3283000 cells/l in f/2 (modyfied) medium, 2683000 cells/l in TMRL medium, 6393000 cells in erlanmeyer with 200 ml volume, 2833000 cells in erlanmeyer with 500 ml volumeand 3600000 cells in TMRL medium in aquarium. Cochlodinium culture with TMRL medium in in erlanmeyer with 100 ml, 3207000 cells/l, 200 ml 3040000 cells/l, 1000 ml 2325000 cells/l counted. The culture of Cochlodiniumin f/2 was possible in the aquarium butthe culture of Cochlodinium in aquarium was not suitable. In f/2 medium the mean of Cochlodiniumin in each treatment was 3600000 cell/lin aquarium. Cochlodinium count in TMRL medium of aquarium was 550000 cell/l. Unsuccessful culture of Cochlodiniumusing Conway medium and Gyllard (f/2-Si) indicated that these mediums weren’t suitablefor Cochlodinium culture. Based on results Cochlodinium count decreased with the increase of erlanmeyer volume in ESM. But, in tubes and in erlanmeyer with volum100 ml, it was almost, successful. We can conclude that Cochlodinium culture required to special condition and medium, that with f/2 (modyfied) medium was gained.

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APA: Copy

GHORBANI VAGHEIE, REZA, ASADI, ALIREZA, & PAIGOZAR, AKBAR. (2013). THE COMPARISON OF DIFFERENT METHOD CULTURES OF HARMFUL DINOFLAGEL COCHLODINIUM POLYKRIKOIDES AT LABORATORY CONDITION. JOURNAL OF MARINE BIOLOGY, 5(19), 79-88. SID. https://sid.ir/paper/174690/en

Vancouver: Copy

GHORBANI VAGHEIE REZA, ASADI ALIREZA, PAIGOZAR AKBAR. THE COMPARISON OF DIFFERENT METHOD CULTURES OF HARMFUL DINOFLAGEL COCHLODINIUM POLYKRIKOIDES AT LABORATORY CONDITION. JOURNAL OF MARINE BIOLOGY[Internet]. 2013;5(19):79-88. Available from: https://sid.ir/paper/174690/en

IEEE: Copy

REZA GHORBANI VAGHEIE, ALIREZA ASADI, and AKBAR PAIGOZAR, “THE COMPARISON OF DIFFERENT METHOD CULTURES OF HARMFUL DINOFLAGEL COCHLODINIUM POLYKRIKOIDES AT LABORATORY CONDITION,” JOURNAL OF MARINE BIOLOGY, vol. 5, no. 19, pp. 79–88, 2013, [Online]. Available: https://sid.ir/paper/174690/en

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